SKU: 32645012309

UA-Glo Luminescent Mycoplasma Detection Kit

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Description

UA-Glo Luminescent Mycoplasma Detection KitProduct Specification Synonyms Mycoplasma luciferase detection kits Stability & Storage Dry ice transport. 20 storage is protected from light, and the validity period is 12 months Background The UA Glo Mycoplasma Detection Kit is used for the qualitative detection of mycoplasma contamination in cell cultures. Over 95% of mycoplasma contamination in cell cultures is caused by Mycoplasma fermentans, M. orale, M. pirum, M. hyorhinis, M. hominis, M.

Product Specification


Synonyms Mycoplasma luciferase detection kits
Stability & Storage

Dry ice transport. -20℃ storage is protected from light, and the validity period is 12 months

Background

The UA-Glo Mycoplasma Detection Kit is used for the qualitative detection of mycoplasma contamination in cell cultures. Over 95% of mycoplasma contamination in cell cultures is caused by Mycoplasma fermentans, M. orale, M. pirum, M. hyorhinis, M. hominis, M. salivarius, M. arginine, and Acholeplasma laidlawii. Live mycoplasmas produce kinases that are not expressed by eukaryotic cells, which can convert ADP into ATP. The Mycoplasma Detection Kit provides ADP and other substrates required for mycoplasma kinases to convert ADP into ATP, and then detects the generated ATP via a luciferase luminescence method to confirm whether there is mycoplasma contamination in the cell culture. This kit can quickly detect the eight aforementioned species as well as many other common live mycoplasma strains.

Components

Size

component

10T

Mycoplasma luciferase detection reagent 0.5ml

Mycoplasma luciferase detection reagent 0.1mL

25T

Mycoplasma luciferase detection reagent 1.25ml

Mycoplasma luciferase detection reagent 0.25mL

50T

Mycoplasma luciferase detection reagent 2.5ml

Mycoplasma luciferase detection reagent 0.5mL


Protocol

1. Sample Preparation for Cell Culture Medium to be Tested

  1. Take 1 mL of cell culture medium from cells in the logarithmic growth phase.

  2. Centrifuge the cell culture medium at room temperature (200 g) for 5 minutes. Transfer approximately 800 μL of the supernatant to a new centrifuge tube, making sure not to include any cell debris at the bottom.

  3. Use the prepared cell culture medium for the mycoplasma detection with luciferase assay.

2. Luciferase Mycoplasma Detection

  1. Add 50 μL of the cell culture medium sample to a white opaque 96-well plate.

  2. Equilibrate the luciferase mycoplasma detection reagent and substrate to room temperature (22°C-25°C), and mix gently.

  3. Add 50 μL of the luciferase mycoplasma detection reagent to the 50 μL of the cell culture medium sample.

  4. Mix by gentle shaking for 10 seconds, incubate at room temperature for 5 minutes, and then read the fluorescence value (Reading 1).

  5. Add 10 μL of the luciferase mycoplasma detection substrate.

  6. Mix by gentle shaking for 10 seconds, incubate at room temperature for 10 minutes, and then read the fluorescence value (Reading 2).

3. Results
The ratio of Reading 2 to Reading 1 is used to determine if mycoplasma contamination is present.

 

The ratio of reading 2 to reading 1

Results Explanation and Recommendations

< 1

No Mycoplasma contamination

1-1.2

Possible Mycoplasma contamination. The cells were isolated and tested again after culture for 24-48hr.

> 1.2

Mycoplasma contamination



Guidelines

  • Aliquot Storage: Aliquot and store the reagents according to the instructions at -20°C to ensure reagent stability.

  • Freeze-Thaw Stability: Reagents stored at -20°C, protected from light, can be freeze-thawed twice without affecting the test results.

  • Sample Collection: Collect samples from cells in the logarithmic growth phase for testing. Newly passaged cells should be tested 24-48 hours after subculture.

  • Luciferase Reaction Sensitivity: The luciferase reaction is sensitive to temperature changes. Reagents and samples should be equilibrated to room temperature (22°C-25°C), and the temperature should remain constant (±1°C) throughout the test.

  • For Research Use Only: This product is intended for research purposes only.

Shipping Notes
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Exchange/Return Notes
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SKU: 32645012309

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Houston, US
★★★★★ 5
Finally a ball my power chewers cannot destroy
Size: Medium
I have two dogs, an American Bully and an American Pit Bull Terrier, and both of them love to chew. Most balls last a very short time around here before they are shredded or missing big chunks. This one has actually held up. They chase it, tug on it and sit and chew on it, and it is still in one piece with no pieces breaking off. One of my favorite things is being able to put food inside. I add some kibble or small snacks, and they will happily sit and work on it for a long time. It keeps them busy, gives them something safe to chew, and they get a little reward while they play. When they are done, I just rinse it out and it is ready to go again. If you have strong jawed dogs that usually destroy toys, this ball is worth trying. Mine really enjoy it and I feel better knowing they finally have a ball that can keep up with them.
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Reviewed in the United States on November 22, 2025
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Jen Siefert
Dallas, US
★★★★★ 5
My dog LOVES this ball
Size: Medium, Size: Medium
Does your dog destroy tennis balls in seconds? Yes? Then your dog NEEDS this ball! It's squishy like a tennis ball, fits in ball launchers (even the cheap ones) and bounces when it hits the ground. My dog has been literally playing with this ball all day and not a single mark on it! The only bad thing is that it does not float. Definitely worth the money! Put it in your cart!
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Reviewed in the United States on March 8, 2026
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Wolf-Haven
Lexington, US
★★★★★ 5
Perfect for ball demolishers
Size: Medium
The dogs love the chuck it brand. I don’t know what it’s made of but they go crazy over them. Will not play with another ball. Wonder if it’s “laced” with an addictive scent. Have pits. Very durable. Not able to chew to pieces. Last for about 6 months before there is a small break. I keep a back up because when the ball goes missing the house is in chaos. 😆 🤪
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Reviewed in the United States on March 27, 2026
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Tatiana
Fort Morgan, US
★★★★★ 5
Most durable ball!
Size: Medium
This are my dogs fav ball 😍 they last forever!
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Reviewed in the United States on April 23, 2026
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Nana
Boise, US
★★★★★ 4
Chuckit medium rebound balls
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My 8 month old German shepherd dog loves these balls. They are easy to throw, they bounce great, they are very chewable! She keeps one in her mouth most of the time. It satisfies her need to chew and she does not chew other things. She drops it in the water and lets the hole fill up and drinks that way. The only problem that has caused is suction in her mouth and difficulty in dislodging it for her. However, she would be lost without it!
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