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Description
Human uPAR ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. 3. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. 4. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against Plasminogen Activator, Urokinase Receptor (uPAR). After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of Plasminogen Activator, Urokinase Receptor (uPAR) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Plasminogen Activator, Urokinase Receptor ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | The urokinase-type plasminogen activator receptor (uPAR), also known as the urokinase receptor or CD87 (Cluster of Differentiation 87), is a protein encoded by the PLAUR gene in humans. It is a multidomain glycoprotein tethered to the cell membrane by a glycosylphosphatidylinositol (GPI) anchor. uPAR was originally identified as a saturable binding site for urokinase (also known as uPA) on the cell surface. uPAR is composed of three tandem LU domains, a member of the three-finger protein family. The structure of uPAR in complex with a peptide antagonist and its native ligand, urokinase, has been solved by X-ray crystallography. uPAR also interacts with several other proteins, including vascular proteins, uPAR-associated protein (uPARAP), and the integrin family of membrane proteins. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.31-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenates and other biological fluids |
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4.5 ★★★★★
Based on 21 reviews
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Product Reviews
★★★★★ 1
Don’t buy
Size: Eufy E25 Omni / E28 Omni E28
Black roller brush doesn’t fit. Quality is not the same as eufy. The filter doesn’t have as many pleats ergo doesn’t filter as well.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 8, 2026
★★★★★ 5
Perfect Fit for Eufy E25 - Great Way to Refresh Your Vacuum
Size: Eufy E25 Omni / E28 Omni E28
This replacement kit worked perfectly with my Eufy E25 robot vacuum/mop. All of the parts fit as expected, and it was nice to be able to swap everything out at once rather than replacing pieces individually.
After installing the new brush, mop, filters, and other components, my machine is running noticeably better. Super easy to do.
The set includes a good assortment of replacement parts, making it a convenient way to refresh your vacuum and keep it working efficiently.
A great value and an easy way to extend the life and performance of your robot vacuum.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 29, 2026
★★★★★ 5
Complete Replacement Set
Size: Eufy E25 Omni / E28 Omni E28
This replacement parts kit is a convenient way to keep a compatible robot vacuum running efficiently without having to source each component separately. The included rolling brush and side brushes help maintain strong cleaning performance by picking up debris effectively, while the filters support proper airflow and dust capture. The dust bags are a useful addition for keeping the self emptying system working smoothly over time. The roller mop also fits well and helps maintain the mopping function without needing frequent replacements. Having a full set like this makes routine maintenance much easier and helps extend the overall lifespan of the vacuum.
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Reviewed in the United States on April 22, 2026
★★★★★ 2
Mop won’t lock and black roller won’t fit
Size: Eufy E25 Omni / E28 Omni E28, Size: Eufy E25 Omni / E28 Omni E28
We’ve had our E28 for about a year now, so it was time to change out some of the more worn parts. This kit looked to have everything I wanted to swap out. It came with two sets of sweeper brushes, two center brushes, the roller mop brush, two dust bin filters and several disposable dust bags.
I was able to get all of the parts to fit on our E28 except the black side main brush. It looks like the diameter of the hole isn’t correct for the model, but the grey side was (see photos and video comparing this set to factory set). The roller mop looked very similar except it had a slightly less high pile. The pieces all seemed good quality over all. Putting the new parts on was easy otherwise and they fit well.
I sent out our E28 for a whole house cleaning and it did well both vacuuming and mopping, or at least on-par with the factory parts with the same noise level. The aim issue was that the roller mop wasn’t locking in place, and when it went to try to wash the mop and popped the whole vacuum out of its base cradle and threw an error a couple times throughout the cleaning. I just had to manually push the roller mop back in and it worked fine until the last wash and then I couldn’t get it to stop throwing the error. So, 2/5 stars for the one piece not fitting and the roller mop having issues during the mop washing process. Guess I’ll have to order from Eufy directly.
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Reviewed in the United States on February 8, 2026
★★★★★ 5
Direct replacment for omni E28!
Size: Eufy E25 Omni / E28 Omni E28, Size: Eufy E25 Omni / E28 Omni E28
This kit is an excellent value and contains direct replacement parts for the Eufy E28 Omni. Everything in the box from the rolling brush and the roller mop to the filters and dust bags matches the original components perfectly in size and fit.
I found that the side brushes and the main roller installed easily without any forcing, and the vacuum performance remained consistent with no loss of suction or strange noises. The HEPA filters fit snugly in the housing, and the dust bags slide right into the docking station just like the brand-name ones. It is much more cost-effective to get this entire bundle than to buy these items individually. If you need to refresh your E28 Omni, this set is a reliable way to get it back to peak performance.
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Reviewed in the United States on January 22, 2026
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