SKU: 32079881893

Mouse Pro-C3 ELISA Kit

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Description

Mouse Pro-C3 ELISA KitProduct Specification Usage Specimen Requirements 1. Specimens should be extracted as soon as possible after collection, following the relevant literature. Experiments should be performed as soon as possible after extraction. If experiments cannot be performed immediately, specimens can be stored at 20C, but repeated freeze thaw cycles should be avoided. 2. Samples containing sodium nitrate (NaN3) cannot be assayed, as NaN3 inhibits horseradish

Product Specification


3. Incubation: Seal the plate with a sealing film and incubate at 37°C for 30 minutes.

4. Prepare the solution: Dilute the 30x concentrated wash solution 30x with distilled water and set aside.

5. Wash: Carefully remove the sealing film, discard the liquid, spin dry, fill each well with wash solution, let it sit for 30 seconds, then discard. Repeat this process 5 times and pat dry.

6. Add enzyme: Add 50 μl of enzyme-labeled reagent to each well, excluding the blank well.

7. Incubation: Perform the same procedure as in step 3.

8. Wash: Perform the same procedure as in step 5.

9. Color Development: Add 50 μl of Color Developing Reagent A to each well, followed by 50 μl of Color Developing Reagent B. Gently shake to mix thoroughly. Incubate at 37°C in the dark for 10 minutes.

10. Stop: Add 50 μl of Stop Solution to each well to terminate the reaction (the blue color will immediately turn yellow).

11. Measurement: Use the blank well as a zero value and measure the absorbance (OD) of each well at 450 nm. Measurements should be performed within 15 minutes after adding the Stop Solution.

Calculation


Draw a standard curve on graph paper with the concentration of the standard as the horizontal axis and the OD value as the vertical axis. Find the corresponding concentration of the sample from the standard curve based on the OD value of the sample; then multiply it by the dilution factor. Alternatively, use the concentration of the standard and the OD value to calculate the linear regression equation of the standard curve, substitute the OD value of the sample into the equation to calculate the sample concentration, and then multiply it by the dilution factor to obtain the actual concentration of the sample.

Standard Curve

Usage

Specimen Requirements


1. Specimens should be extracted as soon as possible after collection, following the relevant literature. Experiments should be performed as soon as possible after extraction. If experiments cannot be performed immediately, specimens can be stored at -20°C, but repeated freeze-thaw cycles should be avoided.

2. Samples containing sodium nitrate (NaN3) cannot be assayed, as NaN3 inhibits horseradish peroxidase (HRP) activity.

Procedure


1. Dilution of Standard: This kit provides one standard sample at full strength. Users can dilute the sample in a small test tube according to the following chart. 10μg/L

Standard No. 5

Add 150μl of the original standard to 150μl of the standard diluent

5μg/L

Standard No. 4

Add 150μl of the original standard to 150μl of the standard diluent

Standard No. 3

Add 150μl of Standard No. 4 to 150μl of Standard Diluent

1.25μg/L

Standard No. 2

Add 150μl of Standard No. 3 to 150μl of Standard Diluent
Species Reactivity Mouse
Theory This kit uses a double-antibody sandwich assay to measure mouse type III collagen propeptide (Pro-C3) levels in samples. A microplate is coated with purified mouse type III collagen propeptide (Pro-C3) antibody to create a solid-phase antibody. Type III collagen propeptide (Pro-C3) is then added sequentially to the antibody-coated microwells. This antibody then binds to HRP-labeled type III collagen propeptide (Pro-C3) antibody, forming an antibody-antigen-enzyme-labeled antibody complex. After thorough washing, the assay is developed with the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of type III collagen propeptide (Pro-C3) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader, and the concentration of mouse type III collagen propeptide (Pro-C3) in the sample is calculated using a standard curve.
Detection Type Used to determine the content of type III collagen propeptide (Pro-C3) in mouse serum, plasma and related liquid samples.
Composition

Serial number

Component name

Specification

Serial number

Component name

Specification

1

30 times concentrated detergent

20ml×1 bottle

6

Developer B solution

6ml×1/bottle

2

Enzyme Labeled Reagent

6ml×1 bottle

7

Stop Solution

6ml×1 bottle

3

Enzyme-coated plate

12 wells ×8 strips

8

Standard (20 μg/L)

0.5 ml ×1 bottle

4

Sample diluent

6 ml ×1 bottle

9

Standard diluent

1.5ml×1 bottle

5

Developer A Liquid

6ml×1 bottle

10

Sealing film

2 photos
General Notes 1. After removing the kit from the refrigerator, allow it to equilibrate at room temperature for 1 hour before use. If the enzyme-coated plate is opened and not completely used, store it in a sealed bag. 2. Crystals may form in the concentrated wash buffer. Warming in a water bath can aid dissolution during dilution. This will not affect the results. 3. Use a pipette for each sample addition step and frequently calibrate its accuracy to avoid experimental error. The time for each addition should ideally be within 5 minutes. For large numbers of samples, using a dispenser is recommended. 4. Develop a standard curve with each measurement, preferably with replicates. If the analyte content in the sample is too high (the sample OD value is greater than the OD value of the first standard well), dilute the sample a certain number of times (n times) with sample diluent before measurement. When calculating the final dilution, multiply by the total dilution factor (×n×5).

5. The sealing film is for single use only to avoid cross contamination.

6. Please store the substrate in a dark place.

7. Strictly follow the instructions for operation. The test results must be determined based on the readings of the microplate reader.

8. All samples, washing solutions and various wastes should be treated as infectious materials.

9. Components of different batches of this reagent must not be mixed.
Storage Temp. Unopened test kit, stored at 2-8°C, has a shelf life of 6 months.
Test Range 0.3μg/L-12μg/L
Shipping Notes
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  • Delivery to the USA:
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Exchange/Return Notes
  • We offer a 30-day return/exchange service after receiving.
  • Final sale items are not eligible for returns or exchanges.
  • To process your return/exchange, please contact us at [email protected]
  • Please click here for more details>>> Return & Exchange Policy
SKU: 32079881893

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4.3 ★★★★★
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Verified Purchase
Robert F
Grantham, US
★★★★★ 4
Not real sweet, but no sugar
I have been taking Metamucil with real sugar regularly for 20+ years. I became concerned about the amount of sugar I was consuming daily and cannot tolerate aspartame. So, I jumped to buy this when I saw Stevia. I quickly noticed that this version is not sweet. It’s sort of bitter. Also, the texture is different due to not having sugar granules mixed in. It also converts to a gel like texture quicker. I later noticed you use less of this product per serving than the one with sugar (read the label!). I have found the best way to take this is to very quickly (seconds) consume the serving after adding water. Don’t wait too long. I down it like taking a shot out of a shot glass followed quickly by a glass of water. After a while, I got used to the flavor. Overall, I am pleased I am consuming much less sugar and really don’t notice the change anymore.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on June 2, 2022
T
Verified Purchase
Tim M.
Bozeman, US
★★★★★ 5
This product is very important to your well being.
The Metamucil is everything I hoped for. If you’re concerned about your sugar intake then I suggest the one with Stevia. Yes the one with natural sugar tastes the best but once you graduate to 3 times a day that’s a lot of sugar. I would follow the directions. Start with once a day then after 3 or 4 days you’ll find twice a day works nice. I’m on my 4th week so a few days ago I started 3 times. I’m very pleased with the way I feel. Think about it, we take care of our cars better than ourselves sometimes. It’s so important to take care of your digestive system. Have a good day. 😁
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 23, 2021
E
Verified Purchase
Elizabeth Rogers
Louisville, US
★★★★★ 5
Great flavor, less calories than real sugar, thickens very quickly.
I usually get the real sugar version of this rather than this one with Stevia. I have it every morning just to supplement my diet with fiber and the version with stevia is only 30 calories vs 80 for the one with real sugar. I mix it with unflavored protein powder in a shaker cup and this one thickens very quickly. So quickly that I add water after every quarter of the container that I drink and re-shake. Otherwise it gets so think it won’t even come out by the time I’m halfway through. Once I figured that out it’s no big deal but makes it so that I won’t take it in the car to drink on the go.
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Reviewed in the United States on October 26, 2019
K
Verified Purchase
Kristen
Dallas, US
★★★★★ 5
IBS C must have!
I have been suffering with OBS C for a very long time now and my doctor through Amazon Care (if you haven't downloaded that app yet, do it now) recommend that I take magnesium and the Align probiotic with breakfast and Metamucil in the evening before bed. I am finally feeling better and have not had any issues using the restroom! I recommend for sure. The taste is meh but I would rather down something that doesn't taste great as long as I feel better anyway!
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Reviewed in the United States on June 2, 2022
D
Verified Purchase
Dawn Johnson
West Palm Beach, US
★★★★★ 5
Great for my tidy mind. Now the cabinet is organized and clean. Space saving too
Size: Left Facing, Size: Left Facing
Easy install. Glides nicely holds the jars without much movement. The labels are wonderful. I am super pleased. We bought 4 left facing for our cabinet. Not done replacing all the jars and such. But you can see a good idea how it will be. The fit out needs perfectly and looks great. I really like how neat and good visibility too. They seem sturdy with all the weight of the filled bottles also.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 1, 2026

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