Pay in installments of $46.03 with
,
and
Shipping Estimate
USA
- USA
- CAN
- USA
- CAN
Ships within 48 hours · Estimated delivery Jul 27 - Aug 1
Promo Codes Available:
For Your Every Summer RSVP, with Code: SUMMER15
Description
Human REPS1 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
|||||||||||||||||||||||||||||||||
| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against RALBP1 Associated Eps Domain Containing Protein 1 (REPS1). After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of RALBP1 Associated Eps Domain Containing Protein 1 (REPS1) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human RALBP1 Associated Eps Domain Containing Protein 1 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
|
|||||||||||||||||||||||||||||||||
| Background | RALBP1-associated Eps domain-containing protein 1, also known as REPS1, is encoded by the REPS1 gene. This gene encodes a signal transducer protein with two EH domains that interact with proteins involved in signal transduction, endocytosis, and cytoskeletal changes. When isolated from clathrin-coated pits, the encoded protein has been found to associate with sectin 1 and Src homology 3 domain-containing growth factor receptor-binding 2-like (endophilin) interacting protein 1. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
|||||||||||||||||||||||||||||||||
| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
- Except Preorder products are shipped in 48 hours.
- Delivery to the USA:
- Standard Shipping : 3-10 business days
- If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
- Please click here for more details>>> Return & Exchange Policy
4.3 ★★★★★
Based on 11 reviews
Sort
Product Reviews
★★★★★ 5
all driven by artificial super intelligence (ASI)
Format: Hardcover
You are peering inside a black hole at a "point" beyond which you cannot see and where no one knows what exists. The point represents a period of time technologically known as Singularity. Even light cannot escape from the point and on the other side it is known only that there is a profound self replicating intelligence greater than our own, all driven by artificial super intelligence (ASI).
Physicist Stephen Hawking writes that "In contrast with our intellect, computers double their performance every eighteen months. So the danger is real that they could develop intelligence and take over the world".
Computer scientist and professor Vernon Vinge writes that "Within 30 years, we will have the technological means to create super human intelligence. Shortly after the human era will be ended".
Our Final Invention is 267 pages of authoritative manuscript that is compelling, fascinating and beyond the fright stage.
The book's author on numerous occasions refers to "we" as if there exists a unified collective engaged in artificial general intelligence(AGI) or artificial super intelligence (ASI). The reality is that some 56 nations are currently in different stages of arcane artificial intelligence designs. They include antagonists such as North Korea, Iran and suicide regimes from the Middle East. Russia, China and the U.S. are the biggest players as is Israel.
The author believes that super computers fueled by nanotechnology will combine to produce ASI trillions of times more powerful than any human academic or intellectual resources. ASI has the potential to eliminate hunger, poverty, disease and even mortality but disruptions of global economies and politics will be in evidence as balance of powers are shifted. Unemployment dynamics will infect bank tellers, retail clerks,
travel agents, loan officers stock brokers....
Computer software designs are so complex, even incomprehensible, that failures are inevitable. The 1986 Chernobyl meltdown, Three Mile Island, and Fukushima were all designed by highly qualified professionals but with complex infrastructures. Under Singularity as computer speeds double with frequency while human intelligence is unchanged, perhaps the musings of Hawking and Vinge will prove to be prescient.
Our Final Invention is 267 pages of a very dark subject which not even a trace of a happy Betty Grable ending is to be found. My time has expired. Perhaps the final words were well expressed by Jaan Tallin, cofounder of Skype: 'A hard-hitting book about the most important topic of this century and possibly beyond---the issue of whether our species can survive. I wish it was science fiction but I know it's not'!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on January 23, 2016
★★★★★ 5
Gripping and Informative, a Must-read
Format: Hardcover
As someone who struggles to finish books in their entirety, I found Our Final Invention by James Barrat highly readable, deeply informative, and utterly gripping. The book contains a powerful message: through competition, distrust, desire and curiosity, humans will inevitably create an artificial intelligence (AI) that rivals or surpasses our own. Thus, it is wise and necessary to invest now in mitigation efforts and potential safeguards -- increased research and advocacy for AI risk and, most importantly, producing friendly AI.
Barrat covers a lot of ground, but his main argument is summarized as follows: Currently, we humans regularly utilize narrow AI technology (technology capable of achieving specific, programmed goals through unassisted human computing -- Siri, Google search, IBM's Watson, etc). We are also experimenting with "black box" tools and techniques (programs where inputs and outputs are understood and measurable, but the processes in between aren't -- genetic algorithms/programming and software that writes better software) and artificial neural networking (ANN), as seen through efforts to reverse engineer the human brain. And, below the surface, there is an ongoing race between world powers (driven mainly by national security, defense, and international business interests) and guided by AI developers to develop and achieve artificial general intelligence (AGI) -- human-level artificial intelligence. The problem is that once AGI is achieved it will be very difficult to manage, and may very well result in the manifestation of artificial super intelligence (ASI) -- greater than human-level intelligence.
ASI could theoretically become thousands of times smarter than the smartest human being alive. It won't think like us, won't want to be ruled by us, and, most crucially, it won't want to be turned off. In fact, ASI would likely regard us as potential fuel for its quest to duplicate and improve itself exponentially in order to achieve its goals.
Throughout the book, Barrat refers to interesting psychological phenomena and concepts (such as the normalcy bias), while drawing on personal experiences, historic events, and interviews with computer programmers, inventors and philosophers, to tactfully illustrate how progress in AI development is dangerously rapid. Adequate checks and balances are not in place to deal with a non-ideal intelligence explosion or hard take-off (AGI quickly leading to ASI).
I highly recommend this book to anyone interested in learning about both human beings and the advancement of machines. I suspect that the prominence of AI, as a research field and topic for discussion, will only increase in time (it already has in recent years -- drones, smart technology, Wall Street high frequency trading (HFT), financial modeling), making Our Final Invention a valuable guide or stepping stone for anyone trying to understand our world and the path of the future.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on July 3, 2014
★★★★★ 5
Great book
Format: Hardcover
We love the FGTEEV books!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 31, 2026
★★★★★ 5
FGTeeV:
Format: Hardcover
The 11 yr old loves it.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 17, 2026
★★★★★ 5
Perfect, hardback!!!
Format: Hardcover
Bought this book for my teens. They love it. Perfect hardback quality for the price. They have the whole series
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 5, 2026