SKU: 27478740132

Mitochondrial Membrane Potential Detection Kit (jc-10)

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Description

Mitochondrial Membrane Potential Detection Kit (jc-10)Product Specification Usage 1. Preparation of JC 10 dyeing working solution: The amount of JC 10 staining working solution required for each well of the six well plate is 1 mL, and the amount of JC 10 staining working solution for other culture vessels is similar; For the cell suspension, 0. 5 mL of JC 10 staining working solution is required for every 500,000 to 1,000,000 cells. Take an appropriate amount of JC 10 (200 ) and dilute JC 10 in the

Product Specification

Usage 1. Preparation of JC-10 dyeing working solution:
The amount of JC-10 staining working solution required for each well of the six-well plate is 1 mL, and the amount of JC-10 staining working solution for other culture vessels is similar; For the cell suspension, 0.5 mL of JC-10 staining working solution is required for every 500,000 to 1,000,000 cells. Take an appropriate amount of JC-10 (200 ×) and dilute JC-10 in the proportion of 8mL of ultrapure water per 50μL of JC-10 (200 ×). Thoroughly dissolve and mix JC-10 with vigorous shaking. Then add 2mL of JC-10 staining buffer (5 ×), and mix well to obtain JC-10 staining working solution.
2. Setting of positive control:
CCCP (10 mM) provided in the kit is recommended to be added to the cell culture medium at a ratio of 1: 1000, diluted to 10 μM, and treated the cells for 20 minutes. Subsequently, JC-10 was loaded according to the following method, and mitochondrial membrane potential was detected. For most cells, the membrane potential of mitochondria will usually be completely lost after 20 minutes of 10 μM CCCP treatment, and it should show green fluorescence after JC-10 staining; However, normal cells should show red fluorescence after staining with JC-10. For specific cells, the concentration and duration of CCCP may be different, and it is necessary to refer to relevant literature to decide.
3. For suspension cells:
(1) Take 100,000 to 600,000 cells and resuspend them in 0.5 mL cell culture medium. The cell culture medium can contain serum and phenol red.
(2) Add 0.5 mL of JC-10 dyeing working solution, reverse it several times and mix well. Incubate in a cell incubator at 37 °C for 20 minutes.
(3) During the incubation period, an appropriate amount of JC-10 staining buffer (1 ×) was prepared according to the ratio of adding 4 mL of distilled water per 1 mL of JC-10 staining buffer (5 ×), and placed in an ice bath.
(4) After the incubation at 37 °C, 600g was centrifuged at 4 °C for 3-4 minutes to precipitate the cells. Discard the supernatant and be careful not to aspirate the cells as much as possible.
(5) Wash twice with JC-10 staining buffer (1 ×): add 1 mL of JC-10 staining buffer (1 ×) to resuspend the cells, centrifuge 600 g at 4 °C for 3-4 minutes, pellet the cells, and discard the supernatant. Add 1 mL of JC-10 staining buffer (1 ×) to resuspend the cells, centrifuge 600g at 4 °C for 3-4 minutes, pellet the cells, and discard the supernatant.
(6) After resuspending with an appropriate amount of JC-10 staining buffer (1 ×), observe with a fluorescence microscope or laser confocal microscope, or detect with a fluorescence spectrophotometer or analyze with a flow cytometer.
4. For adherent cells:
Note: For adherent cells, if you want to use fluorescence spectrophotometer or flow cytometer detection, you can collect the cells first, and refer to the detection method of suspended cells after resuspension.
(1) For one well of a six-well plate, aspirate the culture medium, wash the cells once with PBS or other appropriate solution if necessary according to the specific experiment, and add 1 mL of cell culture medium. The cell culture medium may contain serum and phenol red.
(2) Add 1mL of JC-10 dyeing working solution and mix well. Incubate in a cell incubator at 37 °C for 20 minutes.
(3) During the incubation period, an appropriate amount of JC-10 staining buffer (1 ×) was prepared according to the ratio of adding 4 mL of distilled water per 1 mL of JC-10 staining buffer (5 ×), and placed in an ice bath.
(4) After the incubation at 37 °C, the supernatant was aspirated and washed twice with JC-10 staining buffer (1 ×).
(5) 2 mL of cell culture medium is added, and the culture medium may contain serum and phenol red.
(6) Observe under fluorescence microscope or laser confocal microscope.
5. For purified mitochondria:
(1) The prepared JC-10 staining working solution was diluted 5 times with JC-10 staining buffer (1 ×).
(2) 0.9 mL of 5-fold diluted JC-10 staining working solution was added with 0.1 mL of purified mitochondria with a total protein amount of 10-100 μg.
(3) Detection with a fluorescence spectrophotometer or a fluorescence microplate reader: After mixing, directly use a fluorescence spectrophotometer to time scan (time scan). The excitation wavelength is 485nm and the emission wavelength is 590nm. If a fluorescent microplate reader is used and the excitation wavelength cannot be set to 485 nm, the excitation wavelength can be set in the range of 475 to 520 nm. In addition, fluorescence detection can also be performed with reference to the wavelength setting in step 6 below.
(4) Observation with fluorescence microscope or laser confocal microscope: The method is the same as step 6 below.
6. Fluorescence observation and result analysis:
When detecting JC-10 monomer, the excitation light can be set to 490nm and the emission light can be set to 530nm; When detecting the JC-10 polymer, the excitation light can be set to 525 nm and the emission light can be set to 590 nm. Note that it is not necessary to set the excitation light and the emission light at the maximum excitation wavelength and the maximum emission wavelength when measuring fluorescence here. For observation using a fluorescence microscope, when detecting JC-10 monomer, you can refer to the settings when observing other green fluorescence, such as the settings when observing GFP or FITC; When detecting JC-10 polymer, you can refer to the settings when observing other red fluorescence, such as propidium iodide or Cy3. The appearance of green fluorescence indicates that the mitochondrial membrane potential decreases, and the cell is probably in the early stage of apoptosis. The appearance of red fluorescence indicates that the mitochondrial membrane potential is relatively normal and the state of cells is also relatively normal.


Precautions:
1. JC-10 (200 ×) will solidify and stick to the bottom, wall or cover of the centrifuge tube at lower temperatures such as 4 °C and ice bath. It can be incubated in a water bath at 20 ~ 25 °C for a while until it is completely melted. After use.
2. JC-10 (200 ×) must be fully dissolved and mixed with the ultrapure water provided by the kit before JC-10 staining buffer (5 ×) can be added. Do not prepare JC-10 staining buffer (1 ×) first and then add JC-10 (200 ×), as it will be difficult to fully dissolve JC-10, which will seriously affect subsequent detection.
3. When washing with JC-10 staining buffer (1 ×) after loading JC-10, keep the JC-10 staining buffer (1 ×) at about 4 ℃, and the washing effect at this time is better.
4. After the JC-10 probe is loaded and washed, try to complete the follow-up detection within 30 minutes. Store in ice bath before testing.
5. Please do not prepare all JC-10 staining buffer (5 ×) into JC-10 staining buffer (1 ×). JC-10 staining buffer (5 ×) should be directly used during the use of this kit.
6. If precipitate is found in JC-10 staining buffer (5 ×), it must be completely dissolved before use. To promote dissolution, it can be heated at 37 °C.
7. CCCP is a mitochondrial electron transport chain inhibitor, which is toxic. Please pay attention to careful protection.
8. For your safety and health, please wear a lab coat and disposable gloves.
Beilstein 0
Description

Mitochondrial membrane potential detection kit (JC-10) is a kit that uses JC-10 as a fluorescent probe to rapidly and sensitively detect the changes of mitochondrial membrane potential of cells, tissues or purified mitochondria. It can be used for early apoptosis detection.
JC-10 is an ideal fluorescent probe widely used to detect mitochondrial membrane potential ΔΨm. Cellular, tissue, or purified mitochondrial membrane potentials can be detected. When the mitochondrial membrane potential is high, JC-10 gathers in the matrix of mitochondria and forms a polymer, which can produce red fluorescence; When the mitochondrial membrane potential is low, JC-10 cannot gather in the mitochondrial matrix. At this time, JC-10 is a monomer and can produce green fluorescence. In this way, it is very convenient to detect the change of mitochondrial membrane potential through the change of fluorescence color. The relative ratio of red and green fluorescence is commonly used to measure the ratio of mitochondrial depolarization.
The decrease of mitochondrial membrane potential is a landmark event in the early stage of apoptosis. The decrease of cell membrane potential can be easily detected by the transition from red fluorescence to green fluorescence of JC-10, and the transition from red fluorescence to green fluorescence of JC-10 can also be used as a detection index in the early stage of apoptosis.
The maximum excitation wavelength of JC-10 monomer is 515nm, and the maximum emission wavelength is 529nm; The JC-10 polymer has a maximum excitation wavelength of 585 nm and a maximum emission wavelength of 590 nm. In actual observation, conventional settings for observing red fluorescence and green fluorescence may be used.
This kit provides CCCP as a positive control for inducing a decrease in mitochondrial membrane potential. For samples in six-well plates, this kit can detect a total of 100 samples; For samples in 12 wells, this kit can detect a total of 200 samples.

Product Components:

Component Name Specifications
JC-10 (200 ×) 100 μL/tube, 5 tubes in total
Ultrapure water 90mL
JC-10 Staining Buffer (5 ×) 80mL
CCCP (10 mM) 20 μL




PubChem CID 0
Storage Temp. Dry and protected from light at-20 ℃, shelf life is 12 months. Ultrapure water and JC-10 staining buffer (5 ×) can also be stored at 4 °C.
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SKU: 27478740132

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Erik
Los Angeles, US
★★★★★ 5
Good shorts but they run big
Size: 38, Color: Dusty Olive
I've been losing weight and picked these up in my new size (38). It turns out these are cut for a generous American midsection, and have an elastic fit in the waist, so you can (and probably should!) safely go down a size. I got mine in 34 instead and they fit great.
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Reviewed in the United States on May 22, 2026
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MB
Phoenix, US
★★★★★ 5
Great fit, functional, most comfortable shorts!
Size: 30, Color: Anthracite Camo
These are my go-to shorts, I now have five pair in different camo colors! I suggest one waist size smaller than you normally wear, (I buy a 30 instead of a 32, because they stretch and would be too sloppy if I purchased my regular size). The large open slotted pocket on the left, behind the Velcro flap pocket, is perfect for a normal sized cell phone. Plenty of utility pockets for EDC. Since these stretch slightly to accommodate movement and sitting, they are extremely comfortable. Also, my wife says they are good looking on me, so that gets an extra star!
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Reviewed in the United States on March 24, 2026
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garym
Alexandria, US
★★★★★ 5
great shorts
Size: 38, Color: Gunmetal
excellent shorts. slightly loose around the waist, which i prefer. inseam is perfect just above the knee. light weight and roomy. wash well.
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Reviewed in the United States on May 24, 2026
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Sara
Massapequa, US
★★★★★ 5
The perfect throw-on athletic dress (with built-in shorts!)
Size: Small, Color: Navy Blue
I’ve been loving this athletic dress—it’s one of those pieces that’s both functional and cute, which is hard to find. The fabric is lightweight, breathable, and slightly stretchy, so it’s comfortable whether you’re running errands, going for a walk, or just chasing kids around all day. The built-in shorts are a huge plus. They stay in place well and make it feel more secure than a regular dress, especially on busy days. I also appreciate the pockets—they’re actually usable and don’t feel bulky. The fit is relaxed and flowy, which I personally like because it’s easy and flattering without clinging. The straps are adjustable, so you can customize the fit a bit depending on your height or preference. I’d say it runs true to size for a looser fit. If you want it more fitted, you could size down, but I like the casual, sporty look as is. Overall, this is a great everyday summer staple—super easy to style with sneakers or sandals, and perfect for travel or on-the-go days. I’ll definitely be getting more colors!
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Reviewed in the United States on April 24, 2026
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Amber
Battle Creek, US
★★★★★ 5
Perfect for summer days.
Size: Medium, Color: Deep Gray, Size: Medium, Color: Deep Gray
I absolutely love this little athletic summer dress. I typically wear a size ten and ordered this in a medium and it's a perfect fit. I have the straps short so way it doesn't look baggy. It is super soft and comfortable. It is breathable and a perfect cut and length for me so so I plan on wearing it all during hot summer days. This is very versatile and easy to style by layering different things underneath it. I have a gray and leopard sports bra that is a really close shade of gray so it almost looks like it's one piece. I plan to order the teal as I have a swimsuit top that will match that color spot on. It seems like it is good quality and should hold up well to normal usage. I like that it has really big pockets , but I likely will not use them often lol. I just like the fact that if I need to have pockets they are there.
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Reviewed in the United States on April 15, 2026

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