Pay in installments of $56.25 with
,
and
Shipping Estimate
USA
- USA
- CAN
- USA
- CAN
Ships within 48 hours · Estimated delivery Jul 23 - Jul 28
Promo Codes Available:
For Your Every Summer RSVP, with Code: SUMMER15
Description
Human MMP-9 ELISA KitProduct Specification Species Reactivity Human Theory This kit adopts double antibody sandwich enzyme linked immunosorbent detection technology. Specific anti human MMP 9 antibodies were pre coated on high affinity labeled plates. The standard substance, the sample to be tested and the biotinylated detection antibody are added to the well of the enzyme label plate, and after incubation, the MMP 9 present in the sample binds to the solid phase antibody
Product Specification
| Species Reactivity | Human | |||||||||||||||||||||||||||
| Theory | This kit adopts double antibody sandwich enzyme-linked immunosorbent detection technology. Specific anti-human MMP-9 antibodies were pre-coated on high affinity labeled plates. The standard substance, the sample to be tested and the biotinylated detection antibody are added to the well of the enzyme label plate, and after incubation, the MMP-9 present in the sample binds to the solid phase antibody and the detection antibody to form an immune complex. After washing to remove unbound material, horseradish peroxidase-labeled Streptavidin-HRP was added. After washing, a chromogenic substrate is added to protect the color from light. A stop solution was added to stop the reaction, and the absorbance value was measured at a wavelength of 450 nm (reference calibration wavelength of 540 nm or 570 nm). | |||||||||||||||||||||||||||
| Synonym | 92 kDa gelatinase, 92 kDa type IV collagenase, CLG4B, EC 3.4.24, EC 3.4.24.35, Gelatinase B, GELB, macrophage gelatinase, MANDP2, matrix metallopeptidase 9, matrix metalloproteinase 9, matrix metalloproteinase-9, MMP9, MMP-9, type V collagenase | |||||||||||||||||||||||||||
| Composition |
Please use within the expiration date of the kit
|
|||||||||||||||||||||||||||
| Background | Matrix metalloproteinases (MMPs), also known as matrixins, are members of the zinc-calcium-dependent proteolytic enzyme family, which can break down the extracellular matrix (ECM) and participate in the processing of various molecules in different subcellular environments. MMPs play important functions in numerous physiological processes, such as embryonic development, morphogenesis, reproduction, and tissue remodeling. Also involved in inflammation as well as autoimmune diseases such as arthritis, cancer and cardiovascular diseases. The amount of newly synthesized MMPs is mainly regulated at the transcriptional level, and the proteolytic activity of already existing MMPs is controlled not only by the activity of the zymogen, but also by the inhibition of enzyme activity by endogenous inhibitors, such as α2-macroglobulin, and tissue inhibitors of metalloproteinases (TIMPs). MMP-9 (also known as gelatinase B, 92 kDa type IV collagenase, 92 kDa gelatinase, type V collagenase) is secreted as a proglycosylation enzyme. Activation of the zymogen involves proteolytic removal of the N-terminal precursor region, resulting in the formation of an active enzyme of 82 kDa. Active human MMP-9 has 72% and 74% amino acid sequence homology with mouse and rat MMP-9, respectively. In addition to the zinc binding site, the catalytic domain contains three consecutive fibronectin type II homologous units that bind to gelatin. A proline-rich hinge region links the catalytic domain to the C-terminal heme-like domain. In vitro experiments, after treatment with 4-aminophenyl ethyl acetate (APMA), the enzymogen can not only produce active enzyme, but also produce a C-terminal truncated form with equivalent activity. MMP-9 can degrade components in ECM, especially with very high specificity for denatured collagen (gelatin). MMP-9 also cleaves collagen types III, IV, V, XI, as well as elastin, nestin-1, and vitronectin. MMP-9 can also cleave a variety of chemokines and growth factors (e.g., IL-1β, CXCL8/IL-8, CXCL7, CXCL4, CXCL1, LatentTGF-β, membrane-bound TNF-α, VEGF, and FGFbasic), beta-amyloid, substance P, myelin basic protein. This action may increase or decrease the biological activity of these soluble factors, and may also cause them to be released from association with the ECM. MMP-9 can also trigger signaling pathways through various membrane proteins or induce them to fall off the cell membrane to inhibit signaling pathways (E.g. CD44, E-cadherin, integrin, ICAM-1 and IL-2Rα). MMP-9 is produced by a variety of normal and transformed cells, including neutrophils, monocytes, macrophages, astrocytes, fibroblasts, osteoclasts, chondrocytes, keratinocytes, endothelial cells, and epithelial cells. It affects physiological and pathological angiogenesis and vascular remodeling. Activated neutrophils release the MMP-9 precursor and do not bind to TIMP-1, allowing pro-angiogenic FGF-2 to be released from the ECM. The complex of MMP-9 and TIMP-1 failed to induce the release of FGF-2. Neutrophil-derived MMP-9 can exacerbate the inflammatory response, inducing the release of additional neutrophil MMP-9 by the production of collagen-derived polypeptides. MMP-9 also plays an important role in bone formation and remodeling, methamphetamine-induced behavioral sensitization and response, regulation of neuronal synaptic remodeling, trophoblast invasion during implantation, and activation of serine protease inhibitor α1. MMP-9-mediated shedding of adhesion molecules also has a direct effect on tumor cell invasion. Circulating levels of MMP-9 are elevated in many inflammatory disorders, including the formation of intraluminal thrombus, atherosclerosis, Crohn's disease, hepatitis C virus infection, colorectal cancer, and Duchenne muscular dystrophy. The ratio of MMP-9 to TIMP-1 was elevated in multiple sclerosis sera and cystic fibrous sputum but decreased in cytomegalovirus-infected sera. The level of free MMP-9 and the level of MMP-9 and lipocalin-2/NGAL complex were elevated in the urine of patients with ovarian cancer and patients with uterine urethral tract infection, respectively. |
|||||||||||||||||||||||||||
| General Notes | 1. Please use the kit within the validity period. 2. The components of different kits and different batch kits cannot be mixed. 3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with diluent (1 ×) and re-tested; If the cell culture supernatant sample needs to be distributed and diluted, cell culture medium can be used for other intermediate dilutions except dilution with diluent in the last step. 4. Differences in test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipette, the plate washing technique, the reaction time or temperature, the storage of the kit, etc. 5. The terminating solution in the kit is an acidic solution. Please protect your glasses, hands, face and clothes when using it. 6. For scientific research only, not for in vitro diagnosis. |
|||||||||||||||||||||||||||
| Storage Temp. | Kit unopened, stored at 2-8 °C. | |||||||||||||||||||||||||||
| Test Range | 31.3pg/mL-2000pg/mL |
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
- Except Preorder products are shipped in 48 hours.
- Delivery to the USA:
- Standard Shipping : 3-10 business days
- If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
- Please click here for more details>>> Return & Exchange Policy
4.8 ★★★★★
Based on 23 reviews
Sort
Product Reviews
★★★★★ 3
Fun toy, breaks with rough play
Pattern Name: Assorted, Size: Pack of 2
It's a fun toy and my dog loved it, however he is quite rough so the toy broke at the point where the little plastic loop in the frisbee connects. Good for dogs who are a little more gentle.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on February 17, 2026
★★★★★ 5
Best dog frisbee ever!
Pattern Name: Assorted, Size: Pack of 1
These frisbees are the best. We have had these for several years now they have been through countless catches, tug of war, being peed on (ew), and left out in the blazing sun, freezing cold, snow, ice, and rain. They are faded, yes, but they are perfectly intact without even a loose stitch. The design of them is different from others and the fabric of the frisbee has a slight parachute effect that gives your dog just a tiny bit more time to get those long throws. I would buy them again, but the three we have are still going strong.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 11, 2026
★★★★★ 5
Hyper Pet Tennis Balls for Dogs: Because Every Dog Deserves to Play Fetch Like a Pro
Color: Green, Size: Regular 2.5" - 12 Pack
If your dog had a bucket list, fetching tennis balls would probably be near the top. And if that’s the case, then the Hyper Pet Tennis Balls are their VIP ticket to Fetch World. These little balls of joy are the real deal, perfect for small breeds that are living their best life chasing after something that probably doesn’t need to be chased but is just so throwable.
The 12-pack means you can never lose a ball again...unless, of course, it gets lost in a mysterious “dog dimension” where all the socks go. These 2.5-inch tennis balls are the right size for those dogs that aren’t quite large enough to play with the big leagues. Think of them as the perfectly sized appetizer for the full-on Fetch Feast. Plus, they’re made from non-toxic material, so your pup can gnaw on them all day without worrying about a tummy ache (unless they’re overzealous and gobble up the whole ball...don’t worry, I won’t judge).
If your dog doesn’t love fetch yet, they will after these balls come into their life. They’re designed to be durable enough for constant chomping while still offering that classic “bounce, roll, and fetch” action that gets dogs going.
Pros:
• 12 balls = endless fetch sessions (until the dog gets tired, but let’s be honest, that’s probably never).
• Perfect size for small breeds—no choking hazards, just good ol’ fun.
• Non-toxic materials for guilt-free playtime.
• They squeak. And we all know that squeak is like doggy kryptonite.
• Durability means no more fetching balls that break after three tosses (we’ve all been there).
Cons:
• They might not hold up as well with very heavy chewers (but don’t worry, your dog won’t know the difference until they’ve already swallowed half of one).
• They don’t come in tennis-ball-flavor. If they did, I think we’d all be out of a job.
If you’re looking to upgrade your dog’s fetch game (or just keep them entertained while you catch up on your own Netflix binging), these Hyper Pet Tennis Balls are a solid choice. Plus, your dog will be too busy fetching to judge your questionable snack choices.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on December 13, 2024
★★★★★ 5
Prefect for smaller dogs to play
Color: Green, Size: MINI 1.75" - 12 Pack
The only balls my sato mix will fetch! They are a bit smaller than regular balls and are more comfortable in my dogs smaller mouth (she is about 20 lbs). Great bounce and durability, highly recommend!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 19, 2026
★★★★★ 5
Perfect Size - No Squeakers (yay)
Color: Pink, Size: MINI 1.75" - 4 Pack
My 9 lb. Chi-Mix adores her little toy balls. Perfect for her small size (or a medium dog) and full of bounce. I didn't want squeakers because I knew it would drive me nuts. No squeakers in these balls. Perfect training tool to teach fetch. Perfect toys to have play time with your pooch.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 14, 2026
recommand products
Vartika Singh Georgette Party Anarkali Suit in Sea Green
68.04
Sangeet Wear Grey Color Elegant Embroidered Gown Style Anarkali Suit Net Fabric
99.12
Pink Color Function Wear Embroidered Georgette Fabric Designer Salwar Suit
74.76
Beige Color Net Fabric Festival Wear Embroidered work Glorious Salwar Suit
51.24
Embroidered Work Festival Wear Yellow Color Sober Salwar Suit In Chinon Fabric
40.32