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Description
CHO HCP ELISA KitProduct Specification Theory This method uses one step enzyme linked immunosorbent ELISA. Samples containing CHOK1 HCP can be simultaneously reacted with HRP labeled goat anti CHOK1 antibodies and anti CHOK1 antibodies coated on enzyme plates to finally form a sandwich complex of solid phase antibodies HCP labeled antibodies. Unbound antigen antibodies can be removed by washing the plate. After the TMB substrate is added to the well to complete the
Product Specification
| Theory | This method uses one-step enzyme-linked immunosorbent ELISA. Samples containing CHOK1 HCP can be simultaneously reacted with HRP-labeled goat anti-CHOK1 antibodies and anti-CHOK1 antibodies coated on enzyme plates to finally form a sandwich complex of solid phase antibodies-HCP-labeled antibodies. Unbound antigen antibodies can be removed by washing the plate. After the TMB substrate is added to the well to complete the reaction, the color development is stopped after the stop solution is added, and the OD or absorbance value of the reaction solution at 450/650 nm is read with a microplate reader. The OD value or absorbance value is proportional to the HCP content in the solution. From this, the HCP concentration in the solution can be calculated from the standard curve. | |||||||||||||||||||||||||||||||||||||||||||||
| Description | Host Cell Protein (HCP) secreted by CHO (Chinese Hamster Ovary) host cells is likely to cause contamination of biological products. Failure to adequately remove contaminants in the early stage of drug development may lead to a decrease in drug efficacy or induce an immune response. The kit can quantitatively detect the residue of host cell protein in bioengineering pharmaceuticals with high sensitivity, optimize the process flow and ensure the safety of biological products. Product parameters: 1. Sensitivity: 1ng/mL 2. Detection range: 3-100ng/mL 3. Precision: intra-batch coefficient of variation ≤ 10%, inter-batch coefficient of variation ≤ 15% 4. HCP coverage: more than 80% 5. Specificity: This kit is universal because it can specifically react with CHOK1 HCP without relying on the purification process |
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| Composition |
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| General Notes | The termination solution is 2M sulfuric acid, please handle it carefully to prevent splashes! | |||||||||||||||||||||||||||||||||||||||||||||
| Instructions | This kit is used to quantitatively detect the residual content of CHOK1 host cell protein in a sample. | |||||||||||||||||||||||||||||||||||||||||||||
| Storage Temp. | Components 1 and 2 are stored at ≤-20 °C; Components 5 and 6 are stored at room temperature; Components 3, 4, 7, 8 are stored at 2-8 °C. Valid for 12 months. |
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4.2 ★★★★★
Based on 17 reviews
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Product Reviews
★★★★★ 5
A Beta Worth Rooting For
Format: Kindle
In Spare, Violet Fox flips the omegaverse on its head, giving us a Beta heroine determined to make her mark. Joining the Beta Trials to support her sick father, she's thrown into a pack that doesn't want her, especially the possessive Alphas.
But here's the twist: their sweet Omega turns out to be her scent match. Cue the angst, forbidden tension, and a slow-burn romance that will make your heart ache in the best way.
Violet Fox delivers an emotional, refreshing take on the genre, proving Betas aren't "spares." They're stars.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on January 10, 2025
★★★★★ 5
Beta, Alpha, Omega oh my!
Format: Kindle
Omegas are precious and given to Alphas & their packs... but the Betas want in too. To this end, the Beta government is rolling out its trial of assigning a Beta to each Alpha-Omega pack. But forcing a Beta into a pack where they are not wanted will not end well... Of course, no one expected the Omega to fall for the assigned Beta. Great read and cliffhanger
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Reviewed in the United States on February 15, 2025
★★★★★ 3
A familiar story, just with…..less.
Format: Kindle
So, as other reviewers make clear, this is very similar to Pack Darling and The Beta. It’s much closer aligned with The Beta, in plot and maybe more like Pack Darling with characters.
That being said, I don’t hate this…..but it wasn’t great either. It’s both books mentioned but just….less. Less angst, less emotion, less feeling. The plot feels very half fleshed out, and the “bad guy” feels underwhelming. I didn’t really feel any real emotions from and of the male leads, except maybe Oliver. The others fell sorta flat for me. And Mika makes herself out to be this big bad ass straight outta training and then we never see it from here again with the one fitting room incident as the exception.
SPOILER:
The whole, “Oh, I’m actually probably an Omega, but I don’t wanna be but I do actually wanna be but no one can ever know my secret that I do nothing to hide “ thing fell so flat. She never commutes to believing she was secretly an omega, but also mentions her “secret” a lot. It just felt so manufactured.
I’m intrigued enough to read part 2 and see how the author closes everything out, but this is not one I’ll recommend or ever come back to.
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Reviewed in the United States on February 13, 2024
★★★★★ 5
Good start to a series
Format: Kindle
I delayed reading the series for reasons I don’t remember. But my TBR list is huge so I thought I’d take a shot of this and I was pleasantly surprised. I didn’t think the blurb about it was anything special. But it was a very good book. It took some interesting twists and turns. I am so glad the second book is already out. Because I would not have waited patiently. Very slow burn but good storyline. 🔥🔥/5
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Reviewed in the United States on January 3, 2025
★★★★★ 4
A good read
Format: Kindle
Multiple points of view. 3 Alpha men and an Omega male. She is a Beta in training for a new program placing betas in Alpha/Omega packs. Mila is only doing the program for the money to take care of her dad. She wasn't expecting to fall for a pack but when she sees this packs Omega she is done for. There is just something about him. His Alphas are good looking as well. Too bad she is hiding a secret and their government is acting shady. I liked it and can't wait to see where their story goes.
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Reviewed in the United States on November 14, 2023
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