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Description
Mouse IL-1 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect specimens using EDTA or heparin as an anticoagulant and centrifuge at 1000×g for 15 minutes at 2-8°C within 30 minutes of collection. Remove the supernatant for testing, or store at -20°C or -80°C, but avoid repeated freezing and thawing. 3. Cell culture supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for testing, or store at -20°C or -80°C, but avoid repeated freezing and thawing. Pre-Test Preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the gradient working solution of the standard: Add 1 mL of universal diluent to the lyophilized standard. Let stand for 15 minutes to completely dissolve, then gently mix (concentration is 200 pg/mL). Then dilute to the following concentrations: 200 pg/mL, 100 pg/mL, 50 pg/mL, 25 pg/mL, 12.5 pg/mL, 6.25 pg/mL, 3.125 pg/mL, and 0 pg/mL. Serial dilution method: Take seven EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 200 pg/mL standard working solution into the first EP tube and mix thoroughly to make a 100 pg/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with Interleukin 1 (IL-1) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of Interleukin 1 (IL-1) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Interleukin 1 ELISA kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Interleukin-1 (IL-1) is a cytokine with a molecular weight of 17.5 kDa. It is produced by various cells, including macrophages, monocytes, and lymphocytes, and participates in a variety of tissue responses. It acts not only locally but also systemically, particularly in large fractures. Within hours of BMP implantation, IL-1 is detected in the blood and can release progenomic genomic DNA (PGE2) from the cell membranes of fibroblasts and osteocytes. IL-1 can also induce rejection of allogeneic bone grafts. Immunosuppressants such as glucocorticoids and cyclosporine are potent inhibitors of IL-1 synthesis and cellular responses to IL-1. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 3.12-200 pg/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, cell culture supernatant |
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4.2 ★★★★★
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Product Reviews
★★★★★ 5
☀️ Holy Grail SPF Mist! especially for makeup days 💦
Item Package Quantity: 1, Item Package Quantity: 1
If you hate the feeling of greasy sunscreen but still want serious protection, this is the glow-up you’ve been waiting for. I use this as the last step in my morning skincare and as a top-up over makeup throughout the day. It’s lightweight, non-greasy, and gives a dewy finish without looking oily, just a fresh, hydrated glow ✨
The mist is fine and even (no big droplets or weird stickiness), and it dries down fast. No white cast, no clogged pores, no breakouts. Just SPF 50+ that actually works with your makeup instead of wrecking it. I’ve even used it midday at Disneyland and didn’t burn once, and I’m someone who burns if I think too hard about sunshine 🥵
Helpful hint: Shake it well before spraying, and hold it about 6 inches from your face for a nice even mist. One pass usually does the trick, but I like to go back over my nose and cheeks for extra protection ☁️ It also doubles as a midday makeup refresher, especially when your foundation starts looking a little dry 🧴
I brought this with me on a full weekend trip to Disneyland and it absolutely earned its spot in my park bag 🎒 I was in the sun all day, both days, and didn’t burn at all, even with minimal shade and back-to-back rides 🎢 It layered like a dream over makeup, didn’t feel heavy, and made touch-ups fast and easy in between churros. This mist officially passed the Disneyland test. 🎡☀️💕
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Reviewed in the United States on September 19, 2025
★★★★★ 3
Good but heavy smell
Item Package Quantity: 1
I will keep using this bc it works and the sprayer is sooo nice but the smell is so overwhelming. It’s not a bad smell but it’s just so heavy. I’ll use this one but I don’t see myself repurchasing.
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Reviewed in the United States on May 4, 2026
★★★★★ 4
Good but confusing instructions
It is very glowy. After spraying this on my husband commented on why I was sweating so much 😹 I told him it’s my face mist spf. I have very dry and very sensitive skin and this has not broken me out (yet). I’ve used it for about a week now. I don’t know how much sun protection I’m really getting though. And reapplying it all through out the day, esp in the summer will lead to very very oily looking skin.
It’s also confusing because it says do not spray directly onto face. (?) I just hold my breath and close my eyes tightly before I put my mascara on and spray this. So far it’s been helpful with not making my make up look cakey. There is a smell but it’s not a bad smell, slightly fruity and the smell of spf. If you are sensitive to smells this might not be for you.
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Reviewed in the United States on April 5, 2025
★★★★★ 5
Wonderful!
Item Package Quantity: 1
This makes your skin glow. Smells amazing. I’m older and in my 50s and it is great sun coverage and brightener. I will 100% keep using this.
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Reviewed in the United States on June 7, 2026
★★★★★ 5
Great sun protection!
Sun protection factor: 50 Sun Protection Factor (SPF), Size: 6 Ounce (Pack of 2)
My favorite summer sunscreen protection for myself and my kiddos! Smells good and does what it says. Kids are in and out of the water and mom basks in the sun so it protects us all!
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Reviewed in the United States on April 26, 2026