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Description
Biotin Rat Anti-Mouse Ly-6A/E Antibody (S-3835)Product Specification Host Rat Antigen Ly 6A E Synonyms Lymphocyte antigen 6A 2 6E 1; Ly 6A. 2 Ly 6E. 1; Stem cell antigen 1 (SCA 1); T cell activating protein (TAP) Location Cell membrane Accession P05533Q64253 Clone Number S 3835 Antibody Type Rat mAb Isotype IgG2a,k Application FCM Reactivity Ms Positive Sample C57BL 6 mouse splenocytes Purification Protein G Concentration 0. 2 mg ml Conjugation Biotin Physical Appearance Liquid Storage Buffer PBS
Product Specification
| Host | Rat |
| Antigen | Ly-6A/E |
| Synonyms | Lymphocyte antigen 6A-2/6E-1; Ly-6A.2/Ly-6E.1; Stem cell antigen 1 (SCA-1); T-cell-activating protein (TAP) |
| Location | Cell membrane |
| Accession | P05533、Q64253 |
| Clone Number | S-3835 |
| Antibody Type | Rat mAb |
| Isotype | IgG2a,k |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | C57BL/6 mouse splenocytes |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | Biotin |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Ms |
Background
Ly-6A/E, also known as Sca-1 (stem cell antigen-1), is an 18-kDa glycosylphosphatidylinositol (GPI)-anchored cell surface protein belonging to the murine Ly-6 multigene family, predominantly expressed on multipotent hematopoietic stem cells in adult mouse bone marrow and fetal liver, and is widely used as a marker for isolating these stem cells; beyond its stem cell association, Ly-6A/E is upregulated on activated T and B lymphocytes and appears to participate in T-cell receptor-mediated activation, myoblast proliferation and differentiation, bone formation, and interferon-induced immune responses, with expression levels varying between mouse strains carrying different Ly-6 haplotypes.
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