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Description
Mouse PHF8 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be ultrasonically disrupted or repeatedly frozen and thawed. Finally, the homogenate is centrifuged at 5000×g for 5-10 minutes and the supernatant is collected for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a PHD Finger Protein 8 (PHF8) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of PHD Finger Protein 8 (PHF8) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse PHD Finger Protein 8 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | PHD finger protein 8 (PHF8), also known as histone lysine demethylase PHF8, is encoded by the PHF8 gene. PHF8 belongs to the ferrous and α-ketoglutarate-dependent hydroxylase superfamily and is active as a histone lysine demethylase, selective for dimethyl and monomethyl states. It induces an EMT (epithelial to mesenchymal transition)-like process by upregulating the key EMT transcription factors SNAI1 and ZEB1. Mutations in PHF8 cause Siderius-type X-linked intellectual disability (XLMR). In addition to moderate intellectual disability, Siderius-Hamel syndrome is characterized by facial dysmorphism, cleft lip and/or palate, and, in some cases, microcephaly. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.312-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates and other biological fluids |
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4.2 ★★★★★
Based on 21 reviews
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Product Reviews
★★★★★ 5
Perfect Balance of Comfort, Support, and Style!
Size: 934-Z, Color: Black, Size: 934-Z, Color: Black
I’ve been using this chair for a few weeks now, and I’m genuinely impressed with how much of a difference it’s made in my workspace. The mesh back is one of my favorite features—it offers excellent breathability and prevents that sticky, overheated feeling you sometimes get with solid-back chairs, even after sitting for hours. The seat cushion is firm enough to give proper support but still has enough give to keep me comfortable through long workdays. It feels like the perfect middle ground between soft and supportive.
The adjustable foot ring has been a complete game changer for me. I use a taller desk setup, and having somewhere to rest my feet makes a huge difference in posture and overall comfort. Whether I’m sitting upright for focused work or leaning back to read, it’s always at the right height.
The flip-up armrests are another smart design choice. When I’m typing, I like to have them down for support, but when I’m done, I can flip them up and push the chair neatly under my desk—saving space and keeping things tidy. The casters roll smoothly on both hardwood and rugs, so I can move around easily without scraping the floor or getting stuck. The overall build quality feels solid and sturdy, with no wobbles or creaks.
Assembly was surprisingly quick—everything was clearly labeled, the parts felt durable, and it only took me about 20 minutes from opening the box to sitting in the chair. The sleek, modern look is another big plus—it blends in perfectly with my home office décor without feeling bulky or taking up too much visual space.
If you’re looking for a chair that combines comfort, ergonomic support, durability, and style, I can’t recommend this one enough. It’s equally great for work, studying, or just relaxing at your desk, and it’s definitely been worth every penny.
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Reviewed in the United States on August 12, 2025
★★★★★ 3
OK for the price, but overall comfort is avergae
Size: 934-Z, Color: Mustard
Easy to put together, color (mustard) accurate to images, and while I understand this is not a high-end chair, I feel like it still needs to be rated for its quality.
Pros: the seat cushions are fairly comfortable and it rolls well. Adjustable footrest is easy enough and mostly sturdy.
Cons: For a tall office chair, it doesn't go up high enough. I actually had to lower my standing desk to sit at it, which then isn't where I want it when I'm standing now. It's also a bit wobbly. The seat area's attachment to the post isn't as solid as it should be for a tall chair, so while sitting, it does tend to lean/slope forward and wobble side to side. The lumbar support is not adjustable, so I'm not even really sure what it does. And the armrests are not very comfortable to use. Since they are hard plastic, they are just a bit too rigid to rely on for any comfort. They are basically there to hold on to when you're getting out of the chair.
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Reviewed in the United States on May 29, 2026
★★★★★ 4
Good Value for a Tall Chair
Size: 934W-Z, Color: White
I have received this chair just today and would like to give my first impressions of it.
Height is super adjustable as well as the foot rest. My desk is very tall (~34 inches vs standard ~29 inches) and does not suit a standard height chair. This chair's height is enough to sit comfortably at mybut also still having adjustment to be even higher.
Lumbar support is a manual adjustment that can be adjusted by sliding the lumbar piece up or down and it seems it can be tiled as well.
No reclining feature or slight backrest tilt adjustment. I'm used to my chairs reclining so it feels a little awkward siting in a flat base and straight backrest. I feel like I'm sitting too forward or leaning forward slightly but is probably something I can get use to.
Easy to put together and feels very sturdy. Plastic parts of chair are made from a really sturdy plastic, like playhouse plastic materials. Wheels roll around carpet easily. Mesh seems to be a strong material, though the mesh feels kind itchy on bare skin.
Overall, I think this is a good chair to get if you're needing a tall chair for a good value and can sacrifice no backrest tilt.
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Reviewed in the United States on December 3, 2025
★★★★★ 5
Excellent value for money!
Size: 934W-Z, Color: Black, Size: 934W-Z, Color: Black
I absolutely love this office chair! I needed a chair that could support my standing desk without sacrificing comfort, and this one completely exceeded my expectations. It's incredibly sturdy and durable, well-made, and the height adjustment is super convenient, making it easy to find the perfect fit. Even during long hours of work, the seat is comfortable, and the ergonomic design effectively reduces pressure on my back and legs. I love its clean, modern look—it fits my home office perfectly. Assembly was quick and easy, and for this price, the quality is absolutely fantastic!
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Reviewed in the United States on February 24, 2026
★★★★★ 5
Perfect chair!
Size: 934-Z, Color: Blue, Size: 934-Z, Color: Blue
I have an adjustable-height desk (also purchased from Amazon) and needed a drafting chair for those times when my body gets tired of standing. This chair fits the bill and then some! I put it together myself; it took me about an hour, but would take me about half that next time, since now I know what to do (and what NOT to do!). All the parts were there; there are even spare parts (see photo). All the holes were drilled in exactly the right places, too, which isn't always the case when I buy things from Amazon (my standing desk is a great example). The chair fits me; I'm short (5'3.5"), and often the seats of furniture are too deep for me to rest my feet flat on the floor. The depth of the seat on this chair is just a little over 18 inches, and with the adjustable footrest ring, it's perfect. I don't think a tall or heavy person would fit in it, to be honest. But it's perfect for those of us who are vertically challenged. I love that it glides easily on my hardwood floors and that it turns 360 degrees. The chair comes in numerous luscious colors, so take your pick! It is very sturdy, has good lumbar support, and the seat and seat back are very comfortable. And the arms flip up, which was an absolute must when I was shopping for this chair. I got it on sale, too -- $109.00 -- and I'm delighted with this purchase.
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Reviewed in the United States on April 23, 2026