SKU: 17535841232

Human IL-5 ELISA Kit

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Description

Human IL-5 ELISA KitProduct Specification Description Detection Principle: This kit uses double antibody sandwich ELISA technology. The specific anti human IL 5 capture antibody was pre coated on a high affinity microplate. Add the standard, the sample to be tested and the biotin labeled detection antibody into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The IL 5 in the sample

Product Specification

Description

Detection Principle:

This kit uses double antibody sandwich ELISA technology. The specific anti-human IL-5 capture antibody was pre coated on a high affinity microplate. Add the standard, the sample to be tested and the biotin labeled detection antibody into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The IL-5 in the sample combines with the solid-phase antibody and the detection antibody. After washing sufficiently to remove free and unbound components, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After washing again, TMB chromogenic substrate was added and incubated at room temperature in the dark to develop color. The depth of color response is positively correlated with the concentration of IL-5 in the sample. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value at 450 nm detection wavelength (correction wavelength 570-630 nm).

Detection Type: Double antibody sandwich method

Form: pre coated 96 well plate

Test Sample Type: cell supernatant, serum, plasma

Loading Amount: 100 μ L

Kit Components:A copy of pre coated 96 well plate, standard, IL-5 detection antibody, standard dilution, detection buffer, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.

Sensitivity: 0.76pg/ml

Detection Range: 15.63-1000 pg/ml

Recovery Range: 91-107%

Storage Method: 2-8 ℃

Standard Curve:

Background:

Interleukin 5 (IL-5) is a Th2 cytokine member of hematopoietic family, which is composed of 115 amino acids. It is similar to other members of this cytokine family (IL-3 and GM-CSF)Different, the activated form of this glycoprotein is homodimer. IL-5 is secreted by Th2 cells and mast cells. Its biological activity on B cells and other types of cells shows that IL-5 has an important functional effect on the activity of Th2 cells. IL-5 stimulates B cell growth and increases immunoglobulin secretion by binding to its receptor. It is also a key factor in activating eosinophils. IL-5 has been associated with a variety of allergic diseases, including allergic rhinitis and asthma. A large increase in IL-5 was observed in circulating tissues and tracheal tissues, while sputum eosinophils decreased.

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SKU: 17535841232

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Amy G.
Battle Creek, US
★★★★★ 4
Good buy for the money
This is really nice buy. It was way bigger then I thought it was gonna be. So when we did get it I was surprised. Plus it's nice and soft. Worth the money
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Reviewed in the United States on June 16, 2025
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Lake Worth, US
★★★★★ 5
I love it 💕
It’s noiceee. Not too heavy but not too light. Keeps me warm but not too hot. N it’s a really good size especially for the price. It covers me head to toe. It feels rlly soft and the color is like the picture.
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Reviewed in the United States on July 28, 2025
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William R. Amos
Chelsea, US
★★★★★ 3
It’s ok get what you pay for
Color: Plush-lavender, Size: Throw(50"x60")
Very this throw
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Reviewed in the United States on May 30, 2026
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Mrs. Sag
New York, US
★★★★★ 5
This blanket is stunning, matches my pillows perfectly!
Color: New Sage Green, Size: Twin(60"x80"), Color: New Sage Green, Size: Twin(60"x80")
This blanket is absolutely stunning! It matches perfectly with the matching pillows! I don’t allow my dog on the couch so I got her a bean bag and filled it will foam and covered it with this twin size sage green bubble blanket to match my couch, I love the way it looks and my dog loves the way it feels. The faux fur just looks welcoming. It’s thin, lightweight but super cozy and soft, the bubbles give the blanket texture. Runs true to size, it covers the entire bean bag. Definitely helps my dog relax. Great quality and value, highly recommend!
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Reviewed in the United States on May 7, 2026
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Christine Fonseca
Port Orchard, US
★★★★★ 5
Really soft.
Color: Mustard Yellow Ribbed, Size: Throw(50"x60"), Color: Mustard Yellow Ribbed, Size: Throw(50"x60")
Super colorful and so soft. I purchased the to add some color to my lanai. They are perfect! Warm and cozy for chilly winter nights in Florida. Well worth the money. The size is perfect for cuddling.
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Reviewed in the United States on April 7, 2026

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