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Description
IL-7 Protein, MouseProduct Specification Species Mouse Synonyms IL 7 Accession Q544C8 Amino Acid Sequence Glu26 Ile154 Expression System E. coli Purity 95% by SDS PAGE Endotoxin <1EU g Conjugation Unconjugated Tag No Tag Physical Appearance Lyophilized Powder Storage Buffer Lyophilized from a 0. 2 m filtered concentrated solution in PBS, pH 7. 4, 2 % trehalose. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to
Product Specification
| Species | Mouse |
| Synonyms | IL-7 |
| Accession | Q544C8 |
| Amino Acid Sequence | Glu26-Ile154 |
| Expression System | E.coli |
| Purity | ＞95% by SDS-PAGE |
| Endotoxin | <1EU/μg |
| Conjugation | Unconjugated |
| Tag | No Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4, 2 % trehalose. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage |
This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
|
Protocol
1. Goodwin RG, Lupton S, Schmierer A, et al. 1989. Proc Natl Acad Sci U S A. 86:302-6.
2. Sutherland GR, Baker E, Fernandez KE, et al. 1989. Hum Genet. 82:371-2.
3. Lupton SD, Gimpel S, Jerzy R, et al. 1990. J Immunol. 144:3592-601.
4. Noguchi M, Nakamura Y, Russell SM, et al. 1993. Science. 262:1877-80.
5. Fry TJ, Mackall CL. 2002. Blood. 99:3892-904.
6. Fry TJ, Mackall CL. 2002. J Hematother Stem Cell Res. 11:803-7.
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