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Description
Mouse ESR ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with an Estrogen Receptor (ESR) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the Estrogen Receptor (ESR) content in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Estrogen Receptor ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Estrogen receptors (ERs) are protein molecules that are abundant in cells of target organs. They specifically bind to hormones to form hormone-receptor complexes, enabling the hormones to exert their biological effects. Estrogen receptors are classified into two major categories: classical nuclear receptors, including ERα and ERβ, which are located in the cell nucleus and mediate the genotypic effects of estrogen, exerting "genotypic" regulatory effects by regulating the transcription of specific target genes. Second, membrane receptors, including the membrane components of classical nuclear receptors and G protein-coupled receptors GPER1 (GPR30), Gaq-ER, and ER-X, which belong to the G protein-coupled receptor family. These receptors mediate rapid, non-genotypic effects and exert indirect transcriptional regulatory functions through second messenger systems. Some of these receptors appear to function only locally in the brain. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.312-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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4.7 ★★★★★
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Product Reviews
★★★★★ 5
PlayStation 5 is a beast
Style: PS5 Disc, Configuration: Console only
I never thought I would be a gamer at 62yrs old but after playing the PlayStation 5 at my cousins home & seeing the great graphics ,the choice of all the different types of games available for anyone's & everyone's interest, I had to have one. Are they expensive? Yeppers! But, they are well worth it as this will last for many many years, even if I wind up in a retirement home, this will come with me & I will never be bored. I might drive someone else "Batty" ( if I have a room mate he wont be there for long but thems the breaks kid!) This was purchased at an absolute excellent price & I am very thankful. Delivery was fast & on time. Thank you
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Reviewed in the United States on June 6, 2026
★★★★★ 5
Wish I invested in the Playstation 5 sooner
Style: PS5 Disc, Configuration: Console only
Before buying this I had the Switch 2 and Xbox Series X; however, after buying the Playstation 5 I am blown away right out of the box. The Series X and PS5 are both 4K next generation systems, and though the Series X has the 12 teraflops graphics processing the PS5 looked way batter on my 4K TV. I also like the added functionality of the controller adding to the gaming experience with the touch pad, microphone, and gyroscopic motion control. The console is even turns on automatically by changing TV to the system's HDMI input and puts the TV into gaming mode. That is something the Series X and Switch 2 of course never have done. One down side is the system is notorious for not supporting audio cds which going back the original Playstation in 1994 the brand was always able to support. Plus if you get a cd stuck in the disk drive like what I did recently Sony made it possible to remove the disk without destroying you console in a few easy to follow steps. That saves gamers alot of money in repairs if something like that were to ever occur which is a well thought out solution. With former Xbox exclusives moving to the Playstation 5 I highly recommend adding this system to your collection.
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Reviewed in the United States on March 7, 2026
★★★★★ 5
Color me impressed!
Compact, high quality unit, SUPER easy ink refills, great value, fast print and good color. I've lived with 2 printers and moved them between 5 states. A laser printer for good, fast, low cost prints and an all in one ink jet. It was a total pain, they were both big, both have me headaches, heavy and I needed 2. FINALLY, FINALLY I have 1 printer to replace both, do anything I might need and the ink refills MAKE SENSE! Imagine that?! Haha. A printer that doesn't come with just enough ink to print a test page, but comes with 2 full years with of COLOR ink! This is the way forward and thank you HP for going this route. The idea that buying a new printer is cheaper than buying ink refills warms my heart because there are so many printers that end up in landfills, not to mention how much of a consumer headache it is. I will say that I had concerns regarding color print quality and that it might be messy to refill. I'll put your mind at ease - impressive color print quality, speed and adding ink couldn't be easier! (Idk how this printer would travel while full, but everything seems tight and sealed) Overall I couldn't be happier with the end result. The cost, functions, ease of use and low ongoing ink cost are 100% with it in my opinion. I forgot to mention that I've never found a personal or work printer that was easy to use wirelessly. I'd have a headache adding the printer to my PC or couldn't get a crappy app to work. Once I did, I'd get error codes or a jam... Idk, I'm very techy but that's been my experience. This HP worked without issue and the app was very straightforward. I haven't tried advanced options but it does what I need... It just works! I did try scanning and they was fast and easy as well. I have the printer on a 6ft hutch, so fingers crossed everything continues to work and I won't have to bust out the step ladder 6x like I used to.
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Reviewed in the United States on January 9, 2024
★★★★★ 5
I started my own photo printing shop
Having a printer that performs this well has been great. Setting it up was easy to do with the included instructions. I already have a color laser printer which I use all the time, and you may be wondering why I would go buy an inkjet printer. Photos. You can't print on glossy paper with a laser printer very well. All the photo paper I've purchased clearly says it's for inkjet printers. While this hasn't stopped me from trying. The results have been very poor. Especially if you want to cut the photos to specific sizes. Little bits of toner get everywhere.
I've been putting off getting an inkjet because the cartridge system is too expensive and messy. I got more ink on my hands pulling the stickers off the refills than filling every tank on the HP 7602. This printer has all the features you would expect such as Wi-Fi, flatbed scanner, automatic document feeder, 2-sided printing, and even an ID card copy function. If your business provides financing this is something that would really make things easy for you.
My wife and I print photos from our phone using the HP Smart app or the phone's default printing app and both produce excellent results. The photos are where you would expect them to be, and the quality is as good as getting them from the pharmacy.
Like I said I have a laser printer, but I was curious how this would work just printing some regular documents. It’s not quite as fast obviously but the results are just as good. I love the user interface that is so seamlessly placed into the front bezel. Unless it’s telling you something you would never know it was there. This Printer checks a lot of boxes for me and was 100% worth the investment.
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Reviewed in the United States on January 21, 2024
★★★★★ 1
HP Warranty: Know this before buying; Unit malfunction in less than 5 months
UPDATE: Not even 5 months using this printer and there are problems. 1 - the printer's black ink is not working. A blank paper comes out for documents printed in black ink only. The problem: the black ink is not running through the tube that connects the blank ink tank to the ink cartridge. The tube is totally clear. 2 - The ink cartridge does not move to the center when opening the printer cover, preventing access and removal of the ink cartridge.
HP Tech Support: Spent about an hour with tech support for them to tell me what I already knew - the printer is defective.
HP Warranty: HP is not abiding by the terms of the warranty. According to the standard warranty, HP is to first ship a replacement. To ship the defected unit back, the customer is to use the prepaid shipping label provided by HP and use the box of the replacement unit to ship the defective unit back. Without any advance notification to the customers, HP has indicated to me that this policy does not apply to Smart Tank printers. Smart Tank purchasers are responsible to bear the cost of shipping the defective unit and providing the box. Once HP receives the defective unit, they will then ship the replacement. This policy obviously extends the period the customer is without a working printer. I tried pushing back, but to no avail.
Replaced a HP Officejet Pro 8600 printer. The HP 7602 takes up less space, is well made, looks nice and the printing quality is better. The printing speed is slower, which will be much more noticeable when printing multiple, double sided pages. The paper tray does not have a slider from the top to ensure that the paper stays level and in place. It only has sliders on the side. I would have liked to see an Officejet Pro 8600 equivalent tank printer. The cost of ink cartridges for regular inkjet printers can not be justified for someone that does not print much.
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Reviewed in the United States on March 8, 2025