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Description
GFP Recombinant Rabbit mAb (Alexa Fluor® 647 Conjugate) (S-296-32)Product Specification Host Rabbit Antigen GFP Synonyms Green fluorescent protein Immunogen Synthetic Peptide Accession P42212 Clone Number S 296 32 Antibody Type Recombinant mAb Isotype IgG Application ICC, ICFCM Reactivity Species Independent Purification Protein A Concentration 1 mg ml Conjugation Alexa Fluor 647 Physical Appearance Liquid Storage Buffer PBS, 1% BSA, 0. 3% Proclin 300 Stability & Storage 12 months from date of receipt
Product Specification
| Host | Rabbit |
| Antigen | GFP |
| Synonyms | Green fluorescent protein |
| Immunogen | Synthetic Peptide |
| Accession | P42212 |
| Clone Number | S-296-32 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | ICC, ICFCM |
| Reactivity | Species Independent |
| Purification | Protein A |
| Concentration | 1 mg/ml |
| Conjugation | Alexa Fluor® 647 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| ICC | 1:50 | Species Independent |
| ICFCM | 1:1000 | Species Independent |
Background
The green fluorescent protein (GFP) is a ~27 kDa protein composed of 238 amino acids, originally isolated from the jellyfish Aequorea victoria. Its core structure features a unique β-barrel fold with a central fluorescent chromophore (formed by the autocatalytic cyclization and oxidation of Ser65-Tyr66-Gly67). When excited by blue light at 395 nm (primary peak) or 475 nm (secondary peak), GFP emits green fluorescence at 509 nm without requiring additional cofactors. This property has made GFP a revolutionary tool in molecular and cellular biology, widely used for gene expression reporting, protein localization tracking, and dynamic cell imaging.
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