Pay in installments of $39.68 with
,
and
Shipping Estimate
USA
- USA
- CAN
- USA
- CAN
Ships within 48 hours · Estimated delivery Aug 28 - Sep 2
Promo Codes Available:
For Your Every Summer RSVP, with Code: SUMMER15
Description
Rat IL-35 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. Cell Lysis Buffer: Gently wash adherent cells with ice-cold PBS, then trypsinize and collect cells by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash collected cells three times with ice-cold PBS and resuspend in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and remove the supernatant for analysis. Cell Culture Supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for analysis or store at -20°C or -80°C, avoiding repeated freeze-thaw cycles. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 1000pg/mL). Then dilute to the following concentrations: 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.25pg/mL, 15.625pg/mL, and 0pg/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 1000pg/mL standard working solution into the first EP tube and mix thoroughly to make a 500pg/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
|||||||||||||||||||||||||||||||||
| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with an IL-35 capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the IL-35 content in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Rat | |||||||||||||||||||||||||||||||||
| Synonym | Rat IL-35 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
|
|||||||||||||||||||||||||||||||||
| Background | Interleukin-35 (IL-35) is an anti-inflammatory cytokine of the IL-12 family. As a member of the IL-12 family, IL-35 is produced by a wide range of regulatory lymphocytes and plays a role in immunosuppression. IL-35 can inhibit the development of Th1 and Th17 cells by limiting early T cell proliferation. IL-35 is a dimeric protein composed of the IL-12α and IL-27β chains, encoded by two separate genes, IL12A and EBI3 (Epstein-Barr virus-induced gene 3), respectively. Its receptors consist of the IL-12Rβ2 (part of the IL-12R) and gp130 (part of the IL-27R) chains. In contrast to these two related interleukins, IL-35 can signal through only one of these chains. This has been demonstrated in vivo, as the absence of either receptor chain does not affect the effects of IL-35. On regulatory B cells, IL-35 signals through the IL-12Rβ2 and IL-27Rα subunits. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
|||||||||||||||||||||||||||||||||
| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 15.6-1000 pg/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenate, cell lysate, cell culture supernatant and other biological fluids |
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
- Except Preorder products are shipped in 48 hours.
- Delivery to the USA:
- Standard Shipping : 3-10 business days
- If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
- Please click here for more details>>> Return & Exchange Policy
4.0 ★★★★★
Based on 27 reviews
Sort
Product Reviews
★★★★★ 5
Glad to have Asurion Protection Plan!!! So easy to file a claim and get refunded!!!
I bought my ongoing protection plan about six months ago and had not used it for anything. About the same time, I purchased a small fountain for our front porch. A few weeks ago, the pump stopped working and I did everything I could think of to fix the pump, first check to see if the electrical outlet it had been plugged into still worked and it did. I then poured out all the water, added fresh water, then opened the pump to wash it out and remove any debris it had inside. There was none, and of course by now, the manufacturer's return date ended about four months ago. I was very nervous to submit a claim -- I was afraid I would get rejected, that I would have to pack up and return, or that I would be given 20 questions or more and provide mountains of paperwork to submit the claim. But it was surprisingly simple and easy, the person from Asurion was friendly and did her job incredibly well, my claim was created in their system in less than five minutes and I received a total refund minus the tax (turns out online retailer is not allowed to refund the tax because it was already paid to local and state governments. The cost of the fountain minus the tax was still about $165, which allowed me to upgrade to a much better water pump that had a higher output and also has something called "Burn Protection," ie overheating if the water level gets too low or if the pump gets plugged with debris. The total cost of the new pump was about $25 and has all kinds of amazing features as compared to the small fountain that didn't even have burn protection. I installed the new pump and it's working far better than the one that came with the fountain. Bottom line: I am so glad that I purchased the monthly protection plan from Asurion, and told my family how please I had it. They previously told me it was a waste of money and time because they wouldn't honor the claim. but they did, and I am so elated!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on February 13, 2026
★★★★★ 5
Stress and worry free with asurions complete protect plan
Absolutely thrilled with asurions complete protect plan. Worth every penny. My son is on the spectrum, and is very rough with devices, and I myself, am clumsy and go through devices more than the average person. Asurions comply protect plan, holds true and does exactly as promised. It feels so good to know, that next time a device breaks, I don't have to worry and stress over affording a replacement. Claims take literally 5 minutes to file, and I've not yet had an issue with anything not being covered. The only problem I ever had, was initially finding out how to contact them and file a claim. Which is simple. Don't contact Amazon, go to asurions direct website and follow the prompts. I highly recommend asurions complete protect plan, if you like removing stress from your life, and feeling a bit of security.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 18, 2026
★★★★★ 1
Worst company I’ve ever dealt with
This is probably the strongest version to use publicly because it stays factual, detailed, and angry without sounding unhinged. That’s what usually hits hardest with companies like Asurion because it reads like a documented timeline instead of just rage.
I have never dealt with a more disorganized and concerning claims process in my life than what I experienced with Asurion regarding my DJI Mavic Mini Pro 5 drone claim.
This entire nightmare started before the claim was even filed. For over TWO DAYS, multiple Asurion representatives insisted my product “wasn’t registered” and refused to process my claim. Meanwhile, Asurion’s own phone system literally states: “Great news, you no longer have to register your products.” Their own employees did not understand how their own system worked. I was transferred endlessly between departments, hung up on repeatedly, and even sent to Amazon support despite Amazon having nothing to do with the issue. It finally took speaking to a supervisor on the THIRD DAY for someone to admit they were wrong and manually file the claim.
I mailed my drone on May 4th regarding a serious GPS/compass issue where the drone would unexpectedly drift or take off on its own while recording. Considering this is a nearly $2,000 flying device, this was obviously a major safety concern.
Then things somehow got worse.
On May 5th — less than 24 hours after receiving the drone — I got an email saying it had already shipped back to me. No repair details. No explanation. Nothing.
When I called asking what repairs were actually performed, supervisor “CES” told me there were “no notes” explaining what had been done. She promised she would investigate personally and call me back within 24 hours.
She never called back.
On May 8th, after following up myself, another supervisor finally stated that the technician had “soldered/re-soldered” something on the drone. No explanation of WHAT was soldered. No detailed repair notes. No documentation. Nothing.
This is a drone that had ZERO physical damage and had NEVER been crashed.
I asked for written documentation of the repairs and was refused. I was told to “check the portal,” even though the portal contained absolutely no repair information whatsoever. Another supervisor apologized and promised to email me detailed information immediately.
That never happened either.
At this point, multiple supervisors had promised callbacks, documentation, and escalation reviews — and not a single one followed through.
The drone arrived back while I was still on the phone with Asurion. I powered it on and immediately received compass calibration errors. The drone would not properly calibrate.
So after all this, the drone STILL had issues.
Instead of taking accountability, supervisor “Ryan” accused me of “chasing a reimbursement check,” which is insane considering Asurion themselves only offer two resolutions: repair or reimbursement. He repeatedly insisted that because I “received a service,” the issue was basically over. He refused to acknowledge my concerns regarding undocumented soldering repairs on a nearly $2,000 aircraft and ultimately hung up on me.
Since then, the situation has only become more absurd.
Some supervisors told me the situation was unacceptable and assured me I would likely be reimbursed if I mailed the drone back. Others told me they needed more photos, receipts, and serial numbers — despite the fact that all of this had already been submitted previously and somehow “disappeared” from their system.
One representative demanded photos of a serial number physically located on the drone itself. I explained that DJI stores the serial information digitally through the controller/settings, something Asurion should absolutely know if they repair DJI drones professionally.
That representative then refused to help me further and hung up.
Days later, another representative suddenly approved a return shipping label anyway, making all the previous arguments and delays completely pointless.
Now, after weeks of this chaos, I’ve been told by another supervisor not to even send the drone back yet because they “aren’t sure what they’re going to do.” I was explicitly told they may not repair it and may not reimburse it either.
So to summarize:
* Asurion employees didn’t understand their own registration system.
* I was repeatedly hung up on and transferred around.
* Multiple supervisors promised callbacks and never followed through.
* My nearly $2,000 drone was “repaired” in under 24 hours with vague undocumented soldering work.
* Nobody can tell me exactly what was repaired.
* The drone still had calibration issues immediately after return.
* Supervisors contradicted each other constantly.
* Previously submitted documents mysteriously disappeared.
* I was accused of “chasing a check” for wanting accountability regarding a failed repair.
* I still don’t know whether they plan to repair, reimburse, or do nothing.
This has been one of the most frustrating, unprofessional, and concerning customer service experiences I have ever dealt with.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on January 13, 2026
★★★★★ 5
Fantastic Product
Size: 13"-27", Style: Single, Size: 13"-27", Style: Single
The Wali Single Monitor Desk Mount works better than I expected.
First, the basics. The mount comes well packed and the instructions are very simple to understand. There is so little assembly required that the instructions are almost not needed. Assembly and installation can be done in less than ten minutes (not including cable management if you choose to use that feature). Adjusting everything is easy. Overall quality of the parts and the assembled product are quite good.
I have two monitors that I wanted to mount on a flat desktop. I needed to be able to get the monitors right next to each other and still have the desk flat against the wall. Most double monitor stands will not allow for this unless your monitors are the perfect size for the spread of the arms. The movable arms will usually flex toward the wall if you want the monitors to be edge-to-edge with each other. The result is the desk having to be offset from the wall to accommodate this. By using two single monitor stands, I am able to move the posts to positions that allow the arms to be extended in such a way that everything is flat with the wall.
The other frequently stated issue with most monitor stands is that the arms have hydraulic pistons and/or springs to deal with the weight of the attached monitors so they are easy to move up and down. These pistons and springs are often of poor quality and either fail quickly or don't work as expected from the get-go.
This is not a problem with the Wali Single Monitor Desk Mount. The arm is solidly attached to the 16" pole and only moves up and down by loosening the arm mounting and sliding the arm up or down on the pole. The arm, once set to the desired height, can be move forward, backward, side-to-side and tilt. This makes for a much sturdier setup with no pistons or springs. This system works very well, since I (and I think most people) have little need to move a monitor up or down after the initial installation.
I leave the included Allen wrench stuck in one of the vertical facing screws behind a monitor so that it is always handy but out of sight. If I do need to move the monitors up or down, the tool is right there.
I will buy two more of these for my wife's desk.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on October 3, 2024
★★★★★ 5
Rock-solid tall mount to hold a monitor above a large monitor
Size: 13"-32", Style: Single, Size: 13"-32", Style: Single
I bought the 31.5 model to mount a 27" monitor above a 32" monitor on my desk. This is the first monitor mount I have ever purchased and I am thrilled with the entire mount! The installation instructions were simple to follow. All hardware was clearly notated and packaged by part number. The mount holds the 27" monitor (which isn't all that light) perfectly above my desk monitor and is very stable. Adjustment is easy and effective and It doesn't wobble one bit.
The cable routing clips work well and are literally a snap to install. I used the clamp mount but a screw-down desk mount is included.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on October 31, 2025
recommand products
Rec Iolite Trim in Black Pinhole / Matte Powder White Flange
116.10
Rec Iolite 2" Round Pinhole Adj in Haze Pinhole / Matte Powder White Flange
87.57
Rec Iolite LED Adjustable Slot Aperture in Black Slot Aperture / Black Flange
87.57
Rec Iolite Trim in White Pinhole / White Flange
77.40
Rec Iolite LED Pinhole in Black Pinhole / Matte Powder White Flange
87.57