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Description
FITC Mouse Anti-Mouse CD3 Antibody (S-R447)Product Specification Host Mouse Antigen Mouse CD3 Synonyms T cell surface glycoprotein CD3 zeta chain; T cell receptor T3 zeta chain; CD247; Cd3z; Tcrz Location Cell membrane Accession P24161 Clone Number S R447 Antibody Type Mouse mAb Isotype IgG2b,k Application FCM Reactivity Ms Positive Sample C57BL 6 mouse splenocytes Purification Protein A Concentration 0. 2 mg ml Conjugation FITC Physical Appearance Liquid Storage Buffer PBS, 25% Glycerol, 1%
Product Specification
| Host | Mouse |
| Antigen | Mouse CD3 |
| Synonyms | T-cell surface glycoprotein CD3 zeta chain; T-cell receptor T3 zeta chain; CD247; Cd3z; Tcrz |
| Location | Cell membrane |
| Accession | P24161 |
| Clone Number | S-R447 |
| Antibody Type | Mouse mAb |
| Isotype | IgG2b,k |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | C57BL/6 mouse splenocytes |
| Purification | Protein A |
| Concentration | 0.2 mg/ml |
| Conjugation | FITC |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Ms |
Background
The CD3 protein is a T-cell marker, a complex of four structurally distinct membrane glycoprotein isoforms, 20-50 kDa, comprising extracellular, transmembrane and intracellular domains. CD3 is associated with a heterodimer creating the T-cell receptor (TCR). The CD3 complex is responsible for mediating signal transduction to the internal environment upon antigenic recognition by TCR, causing T-cell proliferation and release of cytokines.
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Exchange/Return Notes
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