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Description
RNase HProduct Specification Synonyms Ribonuclease HIRNase HIRibonuclease HRNase H Amino Acid Sequence Molecular Weight 19 kD Purity 95% by SDS PAGE Conjugation Unconjugated Tag His Tag Physical Appearance Liquid Storage Buffer 50 mM KCl10 mM Tris HCl0. 1 mM EDTA1 mM DTT200 g ml BSA 50% GlycerolpH 7. 4 @ 25C Reconstitution Stability & Storage Store at 25 ~ 15 for 2 years Reference [1] Chan S H , Whipple J M , Dai N ,et al. RNase H based analysis of synthetic
Product Specification
| Synonyms | Ribonuclease HI、RNase HI、Ribonuclease H、RNase H |
| Amino Acid Sequence | / |
| Molecular Weight | 19 kD |
| Purity | ＞95% by SDS-PAGE |
| Conjugation | Unconjugated |
| Tag | His Tag |
| Physical Appearance | Liquid |
| Storage Buffer | 50 mM KCl、10 mM Tris-HCl、0.1 mM EDTA、1 mM DTT、200 µg/ml BSA 50% Glycerol（pH 7.4 @ 25°C） |
| Reconstitution | / |
| Stability & Storage | Store at -25 ~ -15℃ for 2 years |
| Reference |
[1] Chan S H , Whipple J M , Dai N ,et al.RNase H-based analysis of synthetic mRNA 5' cap incorporation[J].RNA (New York, N.Y.), 2022, 28(8):1144-1155. [2] Ilina T V , Brosenitsch T , Sluis-Cremer N ,et al.Retroviral RNase H: Structure, mechanism, and inhibition[J]. 2021. |
Background
Endonuclease that specifically degrades the RNA of RNA-DNA hybrids. RNase H participates in DNA replication; it helps to specify the origin of genomic replication by suppressing initiation at origins other than the oriC locus; along with the 5'-3' exonuclease of pol1, it removes RNA primers from the Okazaki fragments of lagging strand synthesis; and it defines the origin of replication for ColE1-type plasmids by specific cleavage of an RNA preprimer. Involved in production of retron derived msDNA (a branched RNA linked by a 2',5'-phosphodiester bond to a single-stranded DNA).
Components
Storage Solution: 5U/μl RNase H、50 mM KCl、10 mM Tris-HCl、0.1 mM EDTA、1 mM DTT、200 µg/ml BSA 50% Glycerol（pH 7.4 @ 25°C） 10*Reaction Buffer: 500 mM Tris-HCl、750 mM KCl、30 mM MgCl2、100mM DTT(pH 8.3 @ 25°C)
Protocol
Set-up a typical reaction as follows 1）Add the following components in sequence 2）Incubate at 37°C for 20 minutes This reaction can be scaled up according to experimental needs.
Guidelines
1、Both metal ion chelating agent and sulfhydryl sealer can inhibit RNase H activity 2、Heating at 65℃ can be inactivated for 10min
Unit Definition
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