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Description
Mouse IL-4 ELISA KitProduct Specification Theory Double antibody sandwich enzyme linked immunosorbent assay was used in this kit. Specific anti mouse IL 4 antibody was precoated on a high affinity microplate. The standard substance, test sample and biotinylated detection antibody were added into the well of microplate. After incubation, IL 4 present in the sample combined with solid phase antibody and detection antibody to form immune complexes. After washing to remove
Product Specification
| Theory | Double antibody sandwich enzyme-linked immunosorbent assay was used in this kit. Specific anti-mouse IL-4 antibody was precoated on a high-affinity microplate. The standard substance, test sample and biotinylated detection antibody were added into the well of microplate. After incubation, IL-4 present in the sample combined with solid-phase antibody and detection antibody to form immune complexes. After washing to remove the unbound material, the horseradish peroxidase-labeled Streptavidin-HRP was added. After washing, the chromogenic substrate was added and the color was developed in the dark. The reaction was terminated by adding the termination solution, and the absorbance value was measured at 450nm wavelength (reference correction wavelength 540nm or 570nm). | |||||||||||||||||||||||||||
| Composition |
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| Background | Interleukin-4 (IL-4), also known as B cell stimulating factor 1, is a monomeric cytokine of about 18-20 kda that has pleiotropic effects in immune responses. The precursor of mouse IL-4 is 140 amino acids, including a 20-amino acid signal peptide and a 120-amino acid mature chain. IL-4 contains three N-linked glycosylation potential sites, three intra-chain disulphide bonds, and its structure is a bundled tetra-α-spirochetes. Similar to human IL-4, mouse IL-4 has a small selective splicer. "Unlike human IL-4, the expression of this spliceosome is thought to be low and of little significance." The amino acid sequence of mature mouse IL-4 shared 43% and 63% identity with human and rat IL-4, respectively. Studies have shown that the activities of human, mouse and rat IL-4 are species specific. IL - 4 by toward Th2 CD4 + T cells, basophils and mast cells and NKT cells, gamma delta T cells with eosinophilic cells expressed. IL - 4 has played a key role in type 2 immune reaction, can promote the Th2 differentiation and B cell secretory immunoglobulin G1 and immunoglobulin E subtypes. IL-4 causes Th2 differentiation by binding to type I or type II receptor complexes containing IL-4 receptor alpha subunits coupled to the γ chain or IL13Rα1, respectively. IL - 4 by cell surface receptors of different dimers, activate JAK family of protein complex amino acid kinase, cause its cytoplasm C phosphorylation. This triggers the recruitment and phosphorylation of STAT6. The phosphorylation and conformational changes of STAT6 lead to dimerization, nuclear translocation, binding and transcriptional activation of STAT6 target genes, including IL-4, IL-5, IL-13, and Th2-specific cytokines GATA3 and c-Maf. On the function, IL - 4 B cell proliferation, survival, and promote mice immune globulin class into IgG1 and IgE, promote the mast cells, eosinophils, and basophils start and chemokines, promote Th2 phenotype naive CD4 + cells, and promote the epithelial cell proliferation and activation. IL-4 is also an important cytokine in tumor immunology. In early experiments in mice, the researchers found that IL-4 had potent anti-tumor abilities. Mice reject tumors expressing IL-4 and develop long-term antitumor immunity. This may be due to the antiangiogenic effect of IL-4 or its ability to activate, select for CD8+T cells. "Paradoxically, emerging evidence suggests that IL-4 is a tumor-promoting molecule, and therefore, IL-4 is a reverse-acting cytokine." "A possible explanation is that IL-4 induces upregulation of antiapoptotic molecules in tumor cells and downregulation of cytolytic molecules in CD8+T cells." In addition, tumor-produced IL-4 is also thought to act on local tumor-associated macrophages (TAMs) to induce the secretion of cathepsins that promote cell migration. |
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| General Notes | 1. Please use the kit within the validity period. 2. Components of different kits and different batch kits should not be mixed. 3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with (1×) The samples were diluted and retested. "If the cell culture supernatant sample needs to be diluted in a distributed manner, cell culture medium may be used for intermediate dilutions, except for the last step when diluent is used." 4. The difference of the test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipettor, the washing technique, the reaction time or temperature, the storage of the kit, etc. 5. The termination solution in the kit is acidic. Please protect your glasses, hands, face and clothes when using it. 6. For scientific research only, not for in vitro diagnosis. |
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| Storage Temp. | Unopened kit, 2-8° C Storage | |||||||||||||||||||||||||||
| Test Range | 15.6pg/mL-1000pg/mL |
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4.6 ★★★★★
Based on 19 reviews
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Product Reviews
★★★★★ 5
Great product from a great company
Color: Black, Size: Rechargeable
This product works very well. There are several other whips to use also. I absolutely love the cream it makes and that is important so I can enjoy my morning coffee. I used this whisk for quite a while then all of a sudden it stopped working. I wrote to the company to explain. A few days later they sent me a beans new whisk. GREAT item and great company to work with.
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Reviewed in the United States on May 23, 2026
★★★★★ 4
Great Frother - almost too powerful
Color: Black, Size: Rechargeable
I’ve been using the Tyohur InstaWhisk milk frother for a while now, and overall I really like it—but I wouldn’t quite give it 5 stars, and honestly it’s because it might be a little too powerful for its own good.
First, the positives: this frother does an amazing job. The single-button control is super easy to use, and I love that you can adjust the speed just by how much pressure you apply. It feels very intuitive, and you can really dial in the exact texture you want. Whether I’m making a latte or mixing something thicker like a protein drink, it handles everything smoothly.
The performance is seriously impressive. On the lower speeds, it creates nice, silky foam, and when you turn it up, it’s incredibly fast—like café-quality foam in seconds. High-speed frothers like this are designed to create rich foam very quickly, and this one definitely delivers on that promise
That said, the power is also what knocks it down a star for me. If you’re not careful (especially when using higher speeds), it can splash pretty easily. The product description says it’s designed to prevent mess with stable low-RPM operation, and that’s true if you stay on the lower settings—but once you ramp it up, you really need to keep the whisk fully submerged or start slow, or it can send milk flying out of the container
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Reviewed in the United States on May 23, 2026
★★★★★ 5
Great frother and well built
Color: Black, Size: Rechargeable
I don’t usually write reviews, but since I’ve been helped by so many reviews from others, I wanted to share my thoughts on the InstaWhisk Milk Frother.
This is a great frother. It really whips cream into a tasty, rich froth. My wife and I have tried cheaper battery-operated frothers in the past, and while they worked fine at first, they didn’t last very long. The InstaWhisk feels well built and works great.
Pro tip: The frother may be a little too powerful at normal speed, so you might need to lightly “feather” the power button to keep the liquid from overflowing the cup. Once you get used to it, it works really well.
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Reviewed in the United States on June 6, 2026
★★★★★ 5
Great frother/stirrer especially for the price.
Color: Black, Size: Rechargeable
Best one available. Have used cheap ones from the store that work pretty good, but batteries are a pain. This is rechargeable and it has a push button that allows you to start it right away without having to turn it on to start it kind of like a power drill. If you start to press it it goes slow the harder you press the faster it goes. The different attachments are great. We use it for both coffee frothing as well as mixing up electrolyte drinks with water with the included mixing attachment. Crazy powerful and easy to use.
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Reviewed in the United States on May 9, 2026
★★★★★ 5
Strong motor. Works great
Color: Black, Size: Rechargeable
I really like this frother. The fact that it’s variable speed, rechargeable, and can spin at a high rate are all great features.
I use it to blend collagen powder and cream into my coffee every morning. Also sometimes use it to
Blend drinks. Strong motor.
Like that the whisks are removable for cleaning and that it comes with different ones.
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Reviewed in the United States on April 9, 2026