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Description
Human NPHS1 (Nephrin) ELISA KitThe Human (NPHS1) Nephrin ELISA Kit measures Nephrin in samples. The plate has been pre coated with Human Nphs1 antibody. Nphs1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human Nphs1 Antibody is added and binds to Nphs1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated Nphs1 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
The Human (NPHS1) Nephrin ELISA Kit measures Nephrin in samples. The plate has been pre-coated with Human Nphs1 antibody. Nphs1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human Nphs1 Antibody is added and binds to Nphs1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated Nphs1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human Nphs1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
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Exchange/Return Notes
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