SKU: 84213779698

Mouse IL-35 (Interleukin 35) QuickTest ELISA Kit

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Description

Mouse IL-35 (Interleukin 35) QuickTest ELISA KitThe Mouse IL 35 (Interleukin 35) QuickTest ELISA Kit measures IL 35 in Mouse Serum, Plasma, Cell Culture Supernatant, Cell Lysate or Tissue Lysate. This kit was based on sandwich ELISA method. The experiment lasted 120 minutes. Capture antibody was conjugated to an affinity tag that was recognized by a specific antibody coated on the QuickTest plate. Add the Cap Det Ab working solution into each well, then add the standards and pilot samples into

The Mouse IL-35 (Interleukin 35) QuickTest ELISA Kit measures IL-35 in Mouse Serum, Plasma, Cell Culture Supernatant, Cell Lysate or Tissue Lysate. This kit was based on sandwich ELISA method. The experiment lasted 120 minutes. Capture antibody was conjugated to an affinity tag that was recognized by a specific antibody coated on the QuickTest plate. Add the Cap/Det Ab working solution into each well, then add the standards and pilot samples into individual wells. If the sample contains IL-35, a capture antibody-IL-35-biotin-detection antibody complex was formed. After incubation, unbound conjugates were removed by wash buffer. HRP-Streptavidin was added. After washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of IL-35 in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.

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SKU: 84213779698

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Carlos h
Charlottesville, US
★★★★★ 5
Good deal. Pretty durable
Size: Large
Dog loves it! Not as crinkly, but still enjoyed even if smashed a bit.
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Reviewed in the United States on February 22, 2026
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JoanieV.
Battle Creek, US
★★★★★ 3
Great ball but easily broken
Size: Large
I got this ball for my two dogs, one is a 90 pound yellow lab mix and the other 45 pound black lab mix. So far only my bigger dog has played with it and has also busted it. He was playing with it for probably half an hour just chomping down to make it crinkle and crunch. It's quite the loud ball!! I noticed the sound wasn't as loud so I took a peek and noticed my dog basically crushed the inner ball into a much smaller ball. He's still having a good time with it though. It's only a matter of time though that he's going to chew the outer shell because it doesn't make noise like it did.
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Reviewed in the United States on December 31, 2024
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Mari Sned
Dallas, US
★★★★★ 5
Dogs go for this ball every time
Size: Large, Size: Large
My Boston's and Rotti love this ball. I had to get two more. It's great for the Boston's because they can grasp it with the way it's made with the softer rubber. They love the crackle sound. It's just the right size and weight for smaller like the Boston Terrier and Rotti. Easy cleaning, rinses right off.
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Reviewed in the United States on May 13, 2026
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buckzmom
Alexandria, US
★★★★★ 5
It's a Hit a Our House!
Size: Large
My strong chewer is still ..... Well, going strong! He loves his new crackle ball. He enjoys the new sound, the bounce back feeling in his jaws when chews. Mom is happy that pup hasn't put a dent in the toy yet! Price was more than reasonable too. We give it 5 Paws Up!
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Reviewed in the United States on May 25, 2026
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Lisa
Dallas, US
★★★★★ 4
Medium size preferred.
Size: Large
I purchased both the large 4" and the medium 3" balls. My 80 lbs German shepherd much prefers the medium size. Show loves to play fetch with it and its lasted well!
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Reviewed in the United States on May 10, 2026

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