SKU: 83176038230

Mouse PR3-ANCA (proteinase-3 anti-neutrophil cytoplasmic antibody) ELISA Kit

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Description

Mouse PR3-ANCA (proteinase-3 anti-neutrophil cytoplasmic antibody) ELISA KitThe Mouse PR3 ANCA (proteinase 3 anti neutrophil cytoplasmic antibody) ELISA Kit measures PR3 ANCA in Mouse Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antigen Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with antigen. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add

The Mouse PR3-ANCA (proteinase-3 anti-neutrophil cytoplasmic antibody) ELISA Kit measures PR3-ANCA in Mouse Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antigen-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with antigen. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antigen. Then, it binds with PR3-ANCA bound to precoated antigen. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of PR3-ANCA in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.

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SKU: 83176038230

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4.4 ★★★★★
Based on 15 reviews
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Lance M. Shaw
West Palm Beach, US
★★★★★ 5
Awesome quality glow balls
Color: I) 8-Pack (2.5" Balls)
I have bought every glow ball on amazon many times over for my german shepherds. My longest lasting balls have been the chuck-it, they seem to glow longer and not get destroyed but are expensive. These glow balls are our new favorite and not only because of the low price. They hold a good glow longer are heavy duty and fit the chuck-it thrower perfectly. I am stocking up on these. Shipping was also next day so it's 100% buy on these.
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Reviewed in the United States on February 8, 2026
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Verified Purchase
Charlow
Louisville, US
★★★★★ 3
Glow in the Dark Balls
Color: 2-Pack (3" Balls)
These balls are ok, not great. The reason they didn’t receive 5 stars is because they are hard/stiff, harder to throw & heavier than the the “Chuck It” glow in the dark balls. They did not hold up to heavy chewers as well as the aforementioned balls.
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Reviewed in the United States on May 15, 2026
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Verified Purchase
Ladybug27
Battle Creek, US
★★★★★ 5
Best Balls Ever!!!
Size: Medium
These are the best balls ever! We have 2 very active ball loving dogs and these bounce very well and they actually hold up with active play and chewing. They still look brand new after daily use for months!
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Reviewed in the United States on May 15, 2026
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Verified Purchase
David
Alexandria, US
★★★★★ 5
Finally a ball my power chewers cannot destroy
Size: Medium
I have two dogs, an American Bully and an American Pit Bull Terrier, and both of them love to chew. Most balls last a very short time around here before they are shredded or missing big chunks. This one has actually held up. They chase it, tug on it and sit and chew on it, and it is still in one piece with no pieces breaking off. One of my favorite things is being able to put food inside. I add some kibble or small snacks, and they will happily sit and work on it for a long time. It keeps them busy, gives them something safe to chew, and they get a little reward while they play. When they are done, I just rinse it out and it is ready to go again. If you have strong jawed dogs that usually destroy toys, this ball is worth trying. Mine really enjoy it and I feel better knowing they finally have a ball that can keep up with them.
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Reviewed in the United States on November 22, 2025
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Verified Purchase
Jen Siefert
Houston, US
★★★★★ 5
My dog LOVES this ball
Size: Medium, Size: Medium
Does your dog destroy tennis balls in seconds? Yes? Then your dog NEEDS this ball! It's squishy like a tennis ball, fits in ball launchers (even the cheap ones) and bounces when it hits the ground. My dog has been literally playing with this ball all day and not a single mark on it! The only bad thing is that it does not float. Definitely worth the money! Put it in your cart!
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Reviewed in the United States on March 8, 2026

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