Human HSPBETA6/HSP-20 (Heat Shock Protein Beta 6) ELISA Kit

Human HSPBETA6/HSP-20 (Heat Shock Protein Beta 6) ELISA KitThe Human HSPBETA6 HSP 20 (Heat Shock Protein Beta 6) ELISA Kit measures HSP 20 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti HSP20 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add

The Human HSPBETA6/HSP-20 (Heat Shock Protein Beta 6) ELISA Kit measures HSP-20 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti HSP20 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with HSP20 bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of HSP20 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.