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Ships within 48 hours · Estimated delivery Jul 16 - Jul 21
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Description
Porcine PLCBeta1/PI-PLC (Phospholipase C Beta 1, Phosphoinositide Specific) ELISA KitThe Porcine PLCBeta1 PI PLC (Phospholipase C Beta 1, Phosphoinositide Specific) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Porcine PLCBeta1 PI PLC. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Porcine PLCBeta1 PI PLC and Avidin Horseradish
The Porcine PLCBeta1/PI-PLC (Phospholipase C Beta 1, Phosphoinositide Specific) ELISA immunoassay kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Porcine PLCBeta1/PI-PLC. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Porcine PLCBeta1/PI-PLC and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Porcine PLCBeta1/PI-PLC, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Porcine PLCBeta1/PI-PLC. You can calculate the concentration of Porcine PLCBeta1/PI-PLC in the samples by comparing the OD of the samples to the standard curve.
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