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Description
Rat HSD3B1 (3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1) ELISA KitThe Rat HSD3B1 (3 beta hydroxysteroid dehydrogenase Delta 5 >4 isomerase type 1) ELISA Kit measures Hsd3b1 in Rat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti Hsd3b1 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash
The Rat HSD3B1 (3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1) ELISA Kit measures Hsd3b1 in Rat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti Hsd3b1 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with Hsd3b1 bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of Hsd3b1 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.
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