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Description
Human TNF-A (Tumor Necrosis Factor Αlpha) ELISA KitThe Human (TNF A) Tumor Necrosis Factor lpha ELISA Kit measures Tumor Necrosis Factor alpha in samples. The plate has been pre coated with Human TNFA antibody. TNFA present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human TNFA Antibody is added and binds to TNFA in the sample. Then Streptavidin HRP is added and binds to the Biotinylated TNFA antibody. After incubation unbound Streptavidin HRP is washed
The Human (TNF-A) Tumor Necrosis Factor Αlpha ELISA Kit measures Tumor Necrosis Factor alpha in samples. The plate has been pre-coated with Human TNFA antibody. TNFA present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human TNFA Antibody is added and binds to TNFA in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated TNFA antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human TNFA. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
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Exchange/Return Notes
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