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Description
Human ATP5B (Atp Synthase Subunit Beta, Mitochondrial) ELISA KitThe Human (ATP5B) Atp Synthase Subunit Beta, Mitochondrial ELISA Kit measures ATP Synthase subunit beta, mitochondrial in samples. The plate has been pre coated with Human ATP5B antibody. ATP5B present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human ATP5B Antibody is added and binds to ATP5B in the sample. Then Streptavidin HRP is added and binds to the Biotinylated ATP5B antibody. After incubation
The Human (ATP5B) Atp Synthase Subunit Beta, Mitochondrial ELISA Kit measures ATP Synthase subunit beta, mitochondrial in samples. The plate has been pre-coated with Human ATP5B antibody. ATP5B present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human ATP5B Antibody is added and binds to ATP5B in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ATP5B antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human ATP5B. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
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