SKU: 43567910794

bsa fatty acid free cho vero mdck

$134.91 $149.90
Save 10%
No.2 Bestseller on salfueradeti.es >

Pay in installments of $37.48 with ShopPay, AfterPay and Klarna

Shipping Estimate
USA
  • USA
  • CAN

Ships within 48 hours · Estimated delivery Aug 30 - Sep 4

Promo Codes Available:

For Your Every Summer RSVP, with Code: SUMMER15

Description

bsa fatty acid free cho vero mdckPremium fr CHO, Vero, MDCK & sensitive Assays SeamlessBio Bovine Serum Albumin Fatty Acid Free ist speziell aufgereinigt, um gebundene Fettsuren zu entfernen, die in Standard BSA natrlich vorkommen. Mit 98% Reinheit und <5 EU mg Endotoxin ist es die erste Wahl fr Fettsure sensitive Zelllinien (CHO, Vero, MDCK) und Lipid Metabolismus Studien. Fatty Acid Free >99% FA Entfernung durch Charcoal Delipidation Reinheit 98% Hher als Standard BSA Low Endotoxin

Premium für CHO, Vero, MDCK & sensitive Assays

SeamlessBio Bovine Serum Albumin Fatty Acid Free ist speziell aufgereinigt, um gebundene Fettsäuren zu entfernen, die in Standard-BSA natürlich vorkommen. Mit ≥98% Reinheit und <5 EU/mg Endotoxin ist es die erste Wahl für Fettsäure-sensitive Zelllinien (CHO, Vero, MDCK) und Lipid-Metabolismus-Studien.

✓ Fatty Acid Free – >99% FA-Entfernung durch Charcoal-Delipidation
✓ Reinheit ≥98% – Höher als Standard-BSA
✓ Low Endotoxin <5 EU/mg – LAL-tested
✓ Keine Interferenz – Ideal für Lipid-sensitive Assays
✓ AU/NZ/US Origin – BSE/TSE-frei

Warum Fatty Acid Free BSA?

Standard-BSA enthält natürlich gebundene Fettsäuren (~0.5-2 mol FA pro mol BSA), hauptsächlich:

  • Palmitate (C16:0) – Gesättigte Fettsäure
  • Stearate (C18:0) – Gesättigte Fettsäure
  • Oleate (C18:1) – Einfach ungesättigte Fettsäure
  • Linoleate (C18:2) – Mehrfach ungesättigte Fettsäure

Probleme in bestimmten Anwendungen:

  • Zellkultur: CHO, Vero, MDCK sind sensitiv gegenüber exogenen Fettsäuren → veränderte Wachstumsraten, beeinträchtigte Produktqualität
  • Lipid-Metabolismus-Studien: Native FA interferieren mit Messungen von Lipid-Transportern, Beta-Oxidation, Lipoprotein-Metabolismus
  • Hormon-Assays: FA beeinflussen Steroid-Bindung (Testosteron, Estrogen, Cortisol)
  • Drug-Binding-Studies: FA konkurrieren um Bindungsstellen am Albumin

Lösung: Fatty Acid Free BSA eliminiert diese Probleme durch Charcoal-Delipidation (Aktivkohle-Behandlung) → >99% FA-Entfernung.

Technische Spezifikationen

Parameter Spezifikation Test-Methode
Quelle Rinderplasma (AU/NZ/US) COO-Zertifikat
Reinheit ≥98% Elektrophorese
Fettsäure-Gehalt <0.01 mol FA/mol BSA GC-MS (>99% Entfernung)
Endotoxin <5 EU/mg LAL-Test
Albumin-Gehalt ≥98 g/L Bradford/BCA
Monomere ≥95% SEC-HPLC
pH-Wert 6.8-7.5 pH-Meter
Format Lyophilized Powder —
Lagerung Raumtemperatur (versiegelt) —
Haltbarkeit 3 Jahre —

Standard vs. Fatty Acid Free BSA

Eigenschaft Standard BSA Fatty Acid Free BSA
Fettsäure-Gehalt 0.5-2 mol FA/mol BSA <0.01 mol FA/mol BSA
Reinheit ≥96% ≥98%
Endotoxin <10 EU/mg <5 EU/mg
Ideal für Western Blot, ELISA, Protein-Standard CHO/Vero/MDCK, Lipid-Studien

Anwendungen

1. CHO/Vero/MDCK Zellkultur

Warum diese Zelllinien Fatty Acid Free BSA brauchen:

CHO (Chinese Hamster Ovary):

  • Biomanufacturing von monoklonalen Antikörpern (mAbs) und rekombinanten Proteinen
  • Exogene Fettsäuren stören Glykosylierung und Produktqualität
  • FDA/EMA bevorzugen FA-freie Supplements für GMP-Produktion
  • Typische Konzentration: 0.5-2 g/L in serum-freien Medien

Vero (African Green Monkey Kidney):

  • Vaccine Production (Polio, Rabies, Rotavirus, Dengue)
  • WHO-zertifizierte Zelllinie für humane Impfstoffe
  • FA-freie Bedingungen sind essentiell für regulatorische Compliance
  • Verbesserte Virus-Titer bei FA-freiem BSA

MDCK (Madin-Darby Canine Kidney):

  • Influenza-Vaccine Production (saisonale und pandemische Impfstoffe)
  • FDA bevorzugt FA-freie Supplements
  • Höhere HA (Hemagglutinin) Ausbeute

2. Lipid-Metabolismus & Fatty Acid Uptake Studies

Studien zu Lipid-Transportern:

  • CD36 (Fatty Acid Translocase): Uptake-Assays ohne Background durch native FA
  • FATP (Fatty Acid Transport Protein): Spezifische FA-Aufnahme-Studien
  • FABPpm (Plasma Membrane Fatty Acid Binding Protein): Bindungs-Studien

Beta-Oxidation-Assays:

  • Messung der mitochondrialen FA-Oxidation
  • Keine Interferenz durch BSA-gebundene FA
  • Verwendung radioaktiv-markierter FA (z.B. 14C-Palmitat)

Lipoprotein-Metabolismus:

  • HDL/LDL-Studien
  • Apolipoprotein-Bindung
  • CETP (Cholesteryl Ester Transfer Protein) Aktivität

Adipozyten-Differenzierung:

  • 3T3-L1 Präadipozyten → Adipozyten
  • Kontrollierte FA-Zugabe für Lipid-Akkumulation
  • Oil Red O Färbung ohne Background

3. Hormone-Binding Studies

Thyroid Hormone (T3, T4) Binding:

  • FA konkurrieren um Bindungsstellen am Albumin
  • Fatty Acid Free BSA ermöglicht präzise Bindungsstudien
  • Wichtig für Schilddrüsen-Diagnostik

Steroid Hormone Assays:

  • Estrogen, Testosteron, Cortisol, Progesteron
  • FA beeinflussen Protein-Bindung
  • Wichtig für Endokrinologie-Forschung

Drug-Protein Binding Studies:

  • Pharmakokinetik-Studien
  • Kompetition zwischen Drug und FA um Albumin-Bindungsstellen
  • Wichtig für Drug Development

4. Serum-freie Medien Formulierung

  • Präzise Kontrolle über Lipid-Zusammensetzung
  • Gezielte Zugabe definierter Fettsäuren (z.B. Oleic Acid, Linoleic Acid)
  • Reproduzierbare Zellkultur-Bedingungen
  • GMP-konforme Medien-Entwicklung

Verfügbare Volumina

Format Packaging Ideal für
Lyophilized Powder 10 g Kleine Labs, Methodenentwicklung
Lyophilized Powder 50 g Routine-Forschung
Lyophilized Powder 100 g High-throughput Labs
Lyophilized Powder 500 g Bioproduktion, GMP

Andere Volumina & Bulk-Orders: Größere Mengen (≥1 kg) sowie kundenspezifische Formate sind auf Anfrage verfügbar. Kontaktieren Sie uns für ein individuelles Angebot.

Lagerung & Handling

Lagerung:

  • Raumtemperatur (15-25°C), trocken, versiegelt
  • Haltbarkeit: 3 Jahre ab Herstellung
  • Nach Öffnen: Bei 2-8°C lagern, innerhalb 6 Monaten verbrauchen

Auflösen von Pulver:

  1. Langsam in Wasser oder Puffer (PBS, TBS) geben
  2. Vorsichtig schwenken (nicht vortexen)
  3. Bei Raumtemperatur oder 37°C lösen
  4. pH auf 7.0-7.4 einstellen
  5. 0.2 µm sterilfiltrieren für Zellkultur

Wichtig: Vermeiden Sie wiederholtes Einfrieren/Auftauen, da dies zu Proteinaggregation führen kann.

Herkunft & Qualitätssicherung

AU/NZ/US – BSE/TSE-freie Regionen (OIE-zertifiziert)

Jede Charge wird geliefert mit:

  • Certificate of Analysis (CoA) – Vollständige Spezifikationen
  • Certificate of Origin (COO) – AU/NZ/US Herkunft dokumentiert
  • Fatty Acid Analysis (GC-MS) – <0.01 mol FA/mol BSA bestätigt
  • Endotoxin-Test-Zertifikat (LAL) – <5 EU/mg bestätigt
  • BSE/TSE-Freiheitserklärung
  • Safety Data Sheet (SDS)

Warum SeamlessBio für BSA Fatty Acid Free?

  • >99% FA-Entfernung – Charcoal-Delipidation validiert
  • Reinheit ≥98% – Höher als Standard-BSA
  • Low Endotoxin <5 EU/mg – Ideal für Zellkultur
  • BSE/TSE-frei – AU/NZ/US Origin
  • Schnelle EU-Lieferung – Lager in Deutschland, 1-3 Tage
  • Kostenfreie Muster – 10g Test-Volume für qualifizierte Kunden
  • Technischer Support – Bei Fragen zu Protokollen
Shipping Notes
  • Free Standard Shipping on $100+ Orders to the USA.
  • Except Preorder products are shipped in 48 hours.
  • Delivery to the USA:
  1. Standard Shipping : 3-10 business days
  • If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
  • We offer a 30-day return/exchange service after receiving.
  • Final sale items are not eligible for returns or exchanges.
  • To process your return/exchange, please contact us at [email protected]
  • Please click here for more details>>> Return & Exchange Policy
SKU: 43567910794

Discover Niche Categories That Outsell

Top-Converting Item to Boost Your Average Order

4.6 ★★★★★
Based on 9 reviews
Sort
Highest Rating
Newest First
Oldest First
Product Reviews
V
Verified Purchase
Vinto
New York, US
★★★★★ 5
It is mini computer for such a low price. My favorite. Just go for it, you will be amazed!
Color: Black, Style: AE-1000W-1AVDF
I would say this is a mini computer and I keep wondering how could they put these many functions inside this small watch and offering it for 15 dollars.I'm blown away by this wonderful product and this is my favorite watch all the time and I rarely wear my other watches. Isn't this amazing that it got the time for close to 50 cities and I can select 4 of them in T1(Home City), T2, T3 and T4 to easily flip through using the bottom right button. This functionality helped me a lot during my 2 international trips through multiple transits. Before the departure I select all the Cities that I'm transiting through so that I can easily flip to that time zone when I land in that airport and I do not need to rely on airport clock or internet to know the time and calculating the time left for catching my connecting flight. I love this watch especially for this functionality. While scrolling through the time zones for selection the tiny world map in the watch will blink the specific geographical area for that time zone. The capabilities of this watch does not end here, It has lot more to offer. We can program 5 daily or one time alarms into this tiny piece. Stopwatch, Counter, 12 or 24 hour time format, Day light saving ON or OFF which simplifies the time setting when the DST switches. We can set it to signal a beep for every hour. There is digitally displayed manual clock at the top right corner which will display your home country time stored in the T1 timezone. When I go on an international trip, I flip the digital display to local time(T2-T4) and will refer the home city time(T1) in the manual clock. The watch is really durable, light weight, stylish, sporty, water proof, 10 years battery life and comes with a detailed instruction for setting up each and every functionality of this watch.What more can I expect for a 15 dollar watch. My impression to buy this watch happened after seeing one my friends wearing it. I bought one for me and 3 more for giving it as gift. My friend lost it twice and bought the same watch 3rd time which proves how much he also likes it. The last thing that I want to say is, I don't like the fancy brands which charge their customers just for the sake of their brand name or fancy packaging and offer noting more than a normal brand offers. I always prefer quality over show off so I picked this Casio watch. I really appreciate that CASIO believed in quality and functionality and not on fancy packaging. I feel really bad that I'm writing this review after 1.5 years of my purchase and I should have done it long back. I highly recommend this watch for everyone and I'm sure you will never be disappointed.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 23, 2016
J
Verified Purchase
James moore
Phoenix, US
★★★★★ 5
Excellent value
Color: Green, Style: AE1000W-3AV
Nice watch typical Casio quality for a excellent price
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 4, 2026
T
Verified Purchase
Thomas S
Louisville, US
★★★★★ 3
The little watch that tried...and almost succeeded.
Color: Black/Black, Style: AE1000W-1BVCF
The main problem with this watch for me is it tries to do too much and relies heavily on the user. With Day light Savings time not being universal in when it starts, when it ends, and which countries decide to use it, it is a lot of work for some functions Day Light Savings time really hurts this watch and what it tries to do. This Casio watch is priced right and pleasant to look at. It has four main functions\modes. 1 Timekeeping 2 World Time 3 Stop watch 4 Alarm I'll focus on the first 2 as this is where it shines and falls short for me. In TIME KEEPING mode --Works Great You can set 4 different times and tab through them with the bottom right button. This is very useful and quite easy to set up. Upon setting up your local time the next three will be based off that and you only need to set Day Light Savings time on or off accordingly. This is easy to maintain and the world map reflects the time zone you are currently in. In WORLD TIME mode you can quickly tab through over 40 cities around the globe. This is where the watch falls short. You need to set each city depending on their Day Light Savings time. Some cities do not use DST, some start in the middle of March, Some start at end of March, ect.. If you do not mind doing this for each city in WORLD TIME which will require you to look up each of their DST information then this is a nice function. I'm not at all interested in doing this. This is a function an atomic watch would excel at but a manual setting watch it's not worth the effort The rest of the features are more than gimmicks. The world map is functional, especially if you set the DST for each of the cities in WORLD TIME. The analog dial always shows your current time no matter what mode you are in or which of the 4 cities you are on in TIME KEEPING mode.The light is unbalanced but sufficient to light the entire dial. You can change it to 3 seconds from it's factory 1.5 seconds which makes it much more useful. If you want this watch to keep track the current time in four cities it is very easy to set up and works quite well.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 10, 2014
R
Verified Purchase
Rebecca
Battle Creek, US
★★★★★ 5
Well made, high quality wallet
Size: One Size, Color: Brown
I bought this for my husband for Father's Day. It's a beautiful, well made, quality wallet. The stitching is heavy duty and the leather is very nice. It isn't bulky. A few months of having it and it seems to have broken in well.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on November 13, 2025
S
Verified Purchase
Shannon Congdon
Carnegie, US
★★★★★ 5
Good wallet all around!
Size: One Size, Color: Brown
I needed a new wallet, it was here, its durable and going to last a while, it has everything I need it to. Theres two spots for where traditionally cash would go, which is nice, and a lot of spots for cards or IDs! Pretty safe, all I've lost is coins when it's upside down when I have too many so thats a good sign! It did have a 'new leather' smell when I got it, but it has gone away after a while, and it looks great! The leather feels and looks high quality! I didn't even know there were magnets, so I cant review them, woops.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on July 14, 2025

recommand products

Citizen Skyhawk Eco-Drive Funkuhr JY0005-50E JY0005 Promaster Herrenuhr
235.00
Orientieren Sie 2. Generation Bambino klassische automatische FAC00005W0 AC00005W Herrenuhr
115.50
Fossilen Townsman automatische Skelett ME3110 Herrenuhr
187.00
Casio G-Shock S Serie analoge digitale Welt Zeit GMA-S110CM-7A1 Herrenuhr
114.00
Seiko 5 automatische 21 Jewels SNK601 SNK601K1 SNK601K Herrenuhr
180.00